| RRC ID |
51603
|
| 著者 |
Shoji M, Nishioka M, Minato H, Harada K, Kubo M, Fukuyama Y, Kuzuhara T.
|
| タイトル |
Neurotrophic activity of jiadifenolide on neuronal precursor cells derived from human induced pluripotent stem cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Although jiadifenolide has been reported to neurotrophin-like activity in primary cultured rat cortical neurons, it is unknown on that of activity in human neurons. Thus, we aimed to assess neurotrophin-like activity by jiadifenolide in human neuronal cells. We analyzed neuronal precursor cells derived from human induced pluripotent stem cells for microtuble-associated-protein-2 expression by immunofluorescence and western blot, following jiadifenolide treatment. Jiadifenolide promoted dendrite outgrowth, facilitated growth, and prevented death in neuronal cells derived from human induced pluripotent stem cells. Interestingly, jiadifenolide also increased postsynaptic density-95 protein expression suggesting that jiadifenolide promotes neuronal maturation and post-synaptic formation. We demonstrate for the first time that jiadifenolide exhibits neurotrophic effects on human neuronal precursor cells.
|
| 巻・号 |
470(4)
|
| ページ |
798-803
|
| 公開日 |
2016-2-19
|
| DOI |
10.1016/j.bbrc.2016.01.092
|
| PII |
S0006-291X(16)30092-4
|
| PMID |
26809091
|
| MeSH |
Cell Differentiation
Cell Enlargement / drug effects
Cell Proliferation / drug effects
Cell Proliferation / physiology
Cells, Cultured
Dose-Response Relationship, Drug
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / drug effects
Induced Pluripotent Stem Cells / physiology
Nerve Growth Factors / administration & dosage*
Neural Stem Cells / cytology*
Neural Stem Cells / drug effects
Neural Stem Cells / physiology*
Neurogenesis / drug effects
Neurogenesis / physiology*
Sesquiterpenes / administration & dosage*
|
| IF |
2.985
|
| 引用数 |
11
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063) |
