論文 - 詳細
| RRC ID | 51613 |
|---|---|
| 著者 | Yan Y, Yin P, Gong H, Xue Y, Zhang G, Fang B, Chen Z, Li Y, Yang C, Huang Z, Yang X, Ge J, Zou Y. |
| タイトル | Nucleosome Assembly Protein 1-Like 1 (Nap1l1) Regulates the Proliferation of Murine Induced Pluripotent Stem Cells. |
| ジャーナル | Cell Physiol Biochem |
| Abstract |
BACKGROUND/AIMS:To investigate whether nucleosome assembly protein 1-like 1 (Nap1l1) regulates the proliferation of induced pluripotent stem cells (iPSC) and the potential mechanisms. METHODS:Nap1l1-knockdown-iPSC and Nap1l1-overexpression-iPSC were constructed by transfection of lentiviral particles. The proliferation of iPSC was detected by MTT analysis, and cell cycle was analyzed by flow cytometry. RESULTS:Nap1l1 overexpression promoted iPSC proliferation and induced G2/M transition compared to their control iPSC while Nap1l1-knockdown-iPSC dramatically displayed the reduced proliferation and accumulated G2/M phase cells. Further analysis showed that Nap1l1 overexpression in iPSC increased the expression of cyclin B1, downregulated the expression of p21 and p27, while knockdown of Nap1l1 showed the opposite effects. In addition, overexpression of Nap1l1 promoted the phosphorylation of AKT and ERK in iPSC, while knockdown of Nap1l1 inhibited the effects. However, these effects displayed in Nap1l1-overexpression-iPSC were greatly suppressed by the inhibition of AKT or ERK signaling. CONCLUSIONS:The results indicate that Nap1l1 promotes the proliferation of iPSC attributable to G2/M transition caused by downregulation of p27 and p21, and upregulation of cyclin B1, the activation of AKT or ERK is involved in the process. The present study has revealed a novel molecular mechanism involved in the proliferation of iPSC. |
| 巻・号 | 38(1) |
| ページ | 340-50 |
| 公開日 | 2016-1-1 |
| DOI | 10.1159/000438634 |
| PII | 000438634 |
| PMID | 26824453 |
| MeSH | Animals Cell Proliferation Cyclin B1 / metabolism Cyclin-Dependent Kinase Inhibitor p21 / metabolism Cyclin-Dependent Kinase Inhibitor p27 / metabolism Down-Regulation Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors Extracellular Signal-Regulated MAP Kinases / metabolism G2 Phase Cell Cycle Checkpoints Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Mice Nucleosome Assembly Protein 1 / antagonists & inhibitors Nucleosome Assembly Protein 1 / genetics Nucleosome Assembly Protein 1 / metabolism* Proto-Oncogene Proteins c-akt / antagonists & inhibitors Proto-Oncogene Proteins c-akt / metabolism RNA Interference RNA, Small Interfering / metabolism Signal Transduction |
| IF | 5.5 |
| 引用数 | 7 |
| リソース情報 | |
| ヒト・動物細胞 | iPS-MEF-Ng-20D-17(APS0001) |