RRC ID 51661
Author Lai ZW, Bolm L, Fuellgraf H, Biniossek ML, Makowiec F, Hopt UT, Werner M, Keck T, Bausch D, Sorio C, Scarpa A, Schilling O, Bronsert P, Wellner UF.
Title Characterization of various cell lines from different ampullary cancer subtypes and cancer associated fibroblast-mediated responses.
Journal BMC Cancer
Abstract BACKGROUND:Ampullary cancer is a relatively rare form of cancer and usually treated by pancreatoduodenectomy, followed by adjuvant therapy. The intestinal subtype is associated with markedly improved prognosis after resection. At present, only few cell lines are available for in vitro studies of ampullary cancer and they have not been collectively characterized.
METHODS:We characterize five ampullary cancer cell lines by subtype maker expression, epithelial-mesenchymal transition (EMT) features, growth and invasion, drug sensitivity and response to cancer-associated fibroblast conditioned medium (CAF-CM).
RESULTS:On the basis of EMT features, subtype marker expression, growth, invasion and drug sensitivity three types of cell lines could be distinguished: mesenchymal-like, pancreatobiliary-like and intestinal-like. Heterogeneous effects from the cell lines in response to CAF-CM, such as different growth rates, induction of EMT markers as well as suppression of intestinal differentiation markers were observed. In addition, proteomic analysis showed a clear difference in intestinal-like cell line from other cell lines.
CONCLUSION:Most of the available AMPAC cell lines seem to reflect a poorly differentiated pancreatobiliary or mesenchymal-like phenotype, which is consistent to their origin. We suggest that the most appropriate cell line model for intestinal-like AMPAC is the SNU869, while others seem to reflect aggressive AMPAC subtypes.
Volume 16
Pages 195
Published 2016-3-8
DOI 10.1186/s12885-016-2193-5
PII 10.1186/s12885-016-2193-5
PMID 26951071
PMC PMC4782372
MeSH Adult Aged Aged, 80 and over Ampulla of Vater / metabolism* Ampulla of Vater / pathology* Biomarkers, Tumor Cell Line, Tumor Cell Movement Cell Proliferation Epithelial-Mesenchymal Transition Female Fibroblasts / metabolism* Fibroblasts / pathology* Gene Expression Profiling Humans Immunohistochemistry Male Middle Aged Neoplasm Grading Neoplasm Metastasis Neoplasm Staging Neoplasms / metabolism* Neoplasms / mortality Neoplasms / pathology* Neoplasms / therapy Prognosis Proteome Tumor Burden
IF 3.15
Times Cited 5
Resource
Human and Animal Cells TGBC50TKB(RCB1280)