RRC ID 51682
Author Iwanabe Y, Masaki C, Tamura A, Tsuka S, Mukaibo T, Kondo Y, Hosokawa R.
Title The effect of low-intensity pulsed ultrasound on wound healing using scratch assay in epithelial cells.
Journal J Prosthodont Res
Abstract PURPOSE:Low-intensity pulsed ultrasound (LIPUS) is widely used in medical fields because it shortens the time required for biologic wound healing in fracture treatment. Also, in dental fields, LIPUS should be effectively employed for implant treatment. However, most of the relevant reports have been published on its effects on bone formation around implants, and the effects of LIPUS on soft tissue healing remain unclear. In the present study, we examined the effects of LIPUS on soft tissue healing using gingival epithelial cells.
METHODS:Gingival epithelial cells were cultured on a dish, followed by LIPUS exposure at a frequency of 3MHz for 15min. The cells were counted with a hemocytometer, and a scratch assay was conducted by measuring the closing area of the scratch wound using a microscope. Following LIPUS exposure, total RNA was collected for microarray analysis. In addition, real-time PCR was performed to examine the mRNA expression level of integrin α6β4. Furthermore, total protein was collected to examine the protein expression level of integrin α6β4 by western blotting.
RESULTS:The cell count and scratch assay demonstrated that LIPUS exposure promoted cell proliferation and scratch-wound closure. Microarray analysis demonstrated the increased expression levels of adhesion-related genes, including integrin. Real-time PCR analysis demonstrated that LIPUS exposure significantly up-regulated the mRNA expression level of integrin α6β4. Western blotting showed intense staining of integrin α6β4.
CONCLUSION:LIPUS exposure promotes wound closure in the scratch assay and up-regulates the expression level of integrin α6β4 as compared with the control.
Volume 60(4)
Pages 308-314
Published 2016-10-1
DOI 10.1016/j.jpor.2016.03.002
PII S1883-1958(16)30001-9
PMID 27026212
MeSH Animals Cell Adhesion / genetics Cells, Cultured Epithelial Cells / physiology* Gingiva / cytology Integrin alpha6beta4 / genetics Integrin alpha6beta4 / metabolism Mice, Inbred C57BL RNA, Messenger Real-Time Polymerase Chain Reaction Ultrasonic Therapy* Ultrasonic Waves* Up-Regulation Wound Healing* / genetics
IF 2.662
Times Cited 8
Human and Animal Cells GE1(RCB1709)