RRC ID 51748
Author Zhang J, Hiromoto S, Yamazaki T, Niu J, Huang H, Jia G, Li H, Ding W, Yuan G.
Title Effect of macrophages on in vitro corrosion behavior of magnesium alloy.
Journal J Biomed Mater Res A
Abstract The influence of cells on the corrosion behavior of biomedical magnesium alloy is an important but less studied topic, which is helpful for understanding the inconsistent corrosion rates between in vitro and in vivo experiments. In this work, macrophages were directly cultured on Mg-2.1Nd-0.2Zn-0.5Zr (wt %, abbreviated as JDBM) alloy surface for 72 or 168 hours. Macrophages retained good viability and the generation of reactive oxygen species (ROS) was greatly promoted on the alloy. Weight loss, Mg(2+) concentration, and cross-section observation results demonstrated that macrophages accelerated the in vitro corrosion of JDBM. The coverage of cell body did not affect the local thickness of corrosion product layer. The corrosion product layer had a porous inner Mg(OH)2 layer and a dense outer layer mainly composed of O, P, Mg, and Ca. The uniform acceleration of JDBM corrosion was attributed to the omnidirection diffusion of ROS from macrophages. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2476-2487, 2016.
Volume 104(10)
Pages 2476-87
Published 2016-10-1
DOI 10.1002/jbm.a.35788
PMID 27223576
MeSH Alloys / chemistry Alloys / metabolism* Animals Biocompatible Materials / chemistry Biocompatible Materials / metabolism* Cell Survival / drug effects Corrosion Macrophages / cytology Macrophages / drug effects* Macrophages / metabolism Magnesium / chemistry Magnesium / metabolism* Materials Testing Mice RAW 264.7 Cells Reactive Oxygen Species / metabolism
IF 3.525
Times Cited 15
Human and Animal Cells RAW 264(RCB0535)