論文 - 詳細
| RRC ID | 51798 |
|---|---|
| 著者 | Yoshida S, Yamamoto N, Wada N, Tomokiyo A, Hasegawa D, Hamano S, Mitarai H, Monnouchi S, Yuda A, Maeda H. |
| タイトル | GDNF From Human Periodontal Ligament Cells Treated With Pro-Inflammatory Cytokines Promotes Neurocytic Differentiation of PC12 Cells. |
| ジャーナル | J Cell Biochem |
| Abstract |
Glial cell line-derived neurotrophic factor (GDNF) is known to mediate multiple biological activities such as promotion of cell motility and proliferation, and morphogenesis. However, little is known about its effects on periodontal ligament (PDL) cells. Recently, we reported that GDNF expression is increased in wounded rat PDL tissue and human PDL cells (HPDLCs) treated with pro-inflammatory cytokines. Here, we investigated the associated expression of GDNF and the pro-inflammatory cytokine interleukin-1 beta (IL-1β) in wounded PDL tissue, and whether HPDLCs secrete GDNF which affects neurocytic differentiation. Rat PDL cells near the wounded area showed intense immunoreactions against an anti-GDNF antibody, where immunoreactivity was also increased against an anti-IL-1β antibody. Compared with untreated cells, HPDLCs treated with IL-1β or tumor necrosis factor-alpha showed an increase in the secretion of GDNF protein. Conditioned medium of IL-1β-treated HPDLCs (IL-1β-CM) increased neurite outgrowth of PC12 rat adrenal pheochromocytoma cells. The expression levels of two neural regeneration-associated genes, growth-associated protein-43 (Gap-43), and small proline-rich repeat protein 1A (Sprr1A), were also upregulated in IL-1β-CM-treated PC12 cells. These stimulatory effects of IL-1β-CM were significantly inhibited by a neutralizing antibody against GDNF. In addition, U0126, a MEK inhibitor, inhibited GDNF-induced neurite outgrowth of PC12 cells. These findings suggest that an increase of GDNF in wounded PDL tissue might play an important role in neural regeneration probably via the MEK/ERK signaling pathway. J. Cell. Biochem. 118: 699-708, 2017. © 2016 Wiley Periodicals, Inc. |
| 巻・号 | 118(4) |
| ページ | 699-708 |
| 公開日 | 2017-4-1 |
| DOI | 10.1002/jcb.25662 |
| PMID | 27463736 |
| MeSH | Animals Cell Differentiation / physiology Cells, Cultured Cornified Envelope Proline-Rich Proteins / genetics Cytokines / pharmacology Cytokines / physiology* GAP-43 Protein / genetics Gene Expression / drug effects Glial Cell Line-Derived Neurotrophic Factor / pharmacology Glial Cell Line-Derived Neurotrophic Factor / physiology* Humans Interleukin-1beta / pharmacology Interleukin-1beta / physiology MAP Kinase Signaling System / drug effects Male Nerve Regeneration / drug effects Nerve Regeneration / physiology Neurites / drug effects Neurites / physiology Neurons / cytology* Neurons / drug effects Neurons / physiology* PC12 Cells Periodontal Ligament / cytology Periodontal Ligament / injuries Periodontal Ligament / physiology* RNA, Messenger / genetics RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Recombinant Proteins / pharmacology Tumor Necrosis Factor-alpha / pharmacology Tumor Necrosis Factor-alpha / physiology |
| IF | 3.448 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |