論文 - 詳細
| RRC ID | 51804 |
|---|---|
| 著者 | Nagashima S, Kobayashi T, Tanaka T, Tanggis, Jirintai S, Takahashi M, Nishizawa T, Okamoto H. |
| タイトル | Analysis of adaptive mutations selected during the consecutive passages of hepatitis E virus produced from an infectious cDNA clone. |
| ジャーナル | Virus Res |
| Abstract |
To characterize the genomic mutations of hepatitis E virus (HEV) during consecutive passages associated with adaptation to growth in cell culture, a cloned genotype 3 HEV [pJE03-1760F/wt, starting virus (SV)] was passaged 10 times in A549 cells, and the entire genomic sequence of the passage 10 (P10) progeny was determined. Compared to SV, P10 virus possessed two non-synonymous (T2808C and A5054G) and four synonymous mutations (C1213T, T2557C, C3118T and C4435T) in the ORF1. Full-length infectious cDNA clones with a single, double (T2808C and A5054G), or all six mutations, identical to P10, were constructed, and their replication capacity was compared. Four (C1213T, T2557C, T2808C and A5054G) of the six viruses with a single mutation grew more efficiently than SV. The P10 virus propagated more rapidly and grew more efficiently than SV and T2808C+A5054G and reached a higher viral load (95.1- and 8.5-fold, respectively) at 20days post-inoculation. An immunofluorescence analysis revealed that a high percentage (>80%) of cells inoculated with the P10 virus expressed ORF2 proteins, while relatively low percentages (nearly 30% or 5%) inoculated with T2808C+A5054G or SV, respectively, expressed ORF2 proteins. We found that not only non-synonymous but also synonymous HEV mutations are independently associated with increased virus production. |
| 巻・号 | 223 |
| ページ | 170-80 |
| 公開日 | 2016-9-2 |
| DOI | 10.1016/j.virusres.2016.07.011 |
| PII | S0168-1702(16)30391-4 |
| PMID | 27485920 |
| MeSH | Adaptation, Biological / genetics* Amino Acid Substitution Cell Line DNA, Complementary Genome, Viral Hepatitis E virus / classification Hepatitis E virus / genetics* Humans Mutation* Phylogeny RNA, Viral* Reassortant Viruses / genetics Recombination, Genetic Selection, Genetic* Viral Load Virus Cultivation Virus Replication |
| IF | 2.934 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |