Reference - Detail
| RRC ID | 51852 |
|---|---|
| Author | Ogra Y, Tejima A, Hatakeyama N, Shiraiwa M, Wu S, Ishikawa T, Yawata A, Anan Y, Suzuki N. |
| Title | Changes in intracellular copper concentration and copper-regulating gene expression after PC12 differentiation into neurons. |
| Journal | Sci Rep |
| Abstract |
It is suspected that some neurodegenerative diseases are a result of the disturbance of copper (Cu) homeostasis, although it remains unclear whether the disturbance of Cu homeostasis has aberrant effects on neurons. Herein, we investigated Cu metabolism specifically in neurons in terms of changes in the intracellular Cu concentration and the expression of Cu-regulating genes, such as Cu transporters and metallothioneins (MTs), before and after the differentiation of rat pheochromocytoma cells (PC12 cells) into neurons. After the differentiation, Cu and Zn imaging with fluorescent probes revealed an increase in intracellular Cu concentration. The concentrations of other essential metals, which were determined by an inductively coupled plasma mass spectrometer, were not altered. The mRNA expression of the Cu influx transporter, Ctr1, was decreased after the differentiation, and the differentiated cells acquired tolerance to Cu and cisplatin, another substrate of Ctr1. In addition, the expression of MT-3, a brain-specific isoform, was increased, contrary to the decreased expression of MT-1 and MT-2. Taken together, the differentiation of PC12 cells into neurons induced MT-3 expression, thereby resulting in intracellular Cu accumulation. The decrease in Ctr1 expression was assumed to be a response aimed at abolishing the physiological accumulation of Cu after the differentiation. |
| Volume | 6 |
| Pages | 33007 |
| Published | 2016-9-13 |
| DOI | 10.1038/srep33007 |
| PII | srep33007 |
| PMID | 27623342 |
| PMC | PMC5020689 |
| MeSH | Animals Cation Transport Proteins / genetics Cation Transport Proteins / metabolism Cell Differentiation Copper / metabolism* Copper Transporter 1 Gene Expression Homeostasis Metallothionein / genetics Metallothionein / metabolism Metallothionein 3 Metals / toxicity Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neurons / cytology* Neurons / drug effects Neurons / metabolism* PC12 Cells RNA, Messenger / genetics RNA, Messenger / metabolism Rats Zinc / metabolism |
| IF | 3.998 |
| Times Cited | 16 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | PC-12(RCB0009) |