Reference - Detail
| RRC ID | 51921 |
|---|---|
| Author | Podyma-Inoue KA, Moriwaki T, Rajapakshe AR, Terasawa K, Hara-Yokoyama M. |
| Title | Characterization of Heparan Sulfate Proteoglycan-positive Recycling Endosomes Isolated from Glioma Cells. |
| Journal | Cancer Genomics Proteomics |
| Abstract |
BACKGROUND:Heparan sulfate proteoglycans (HSPGs)-dependent endocytic events have been involved in glioma progression. Thus, comprehensive understanding of the intracellular trafficking complexes formed in presence of HSPGs would be important for development of glioma treatments. MATERIALS AND METHODS:Subcellular fractionation was used to separate vesicles containing HSPGs from the rat C6 glioma cell line. Isolated HSPG-positive vesicles were further characterized with liquid chromatography-mass spectrometry. RESULTS:The HSPG-positive vesicular fractions, distinct from plasma membrane-derived material, were enriched in endocytic marker, Rab11. Proteomic analysis identified more than two hundred proteins to be associated with vesicular membrane, among them, over eighty were related to endosomal uptake, recycling or vesicular transport. CONCLUSION:Part of HSPGs in glioma cells is internalized through clathrin-dependent endocytosis and undergo recycling. The development of compounds regulating HSPG-mediated trafficking will likely enable design of effective glioma treatment. |
| Volume | 13(6) |
| Pages | 443-452 |
| Published | 2016-11-12 |
| DOI | 10.21873/cgp.20007 |
| PII | 13/6/443 |
| PMID | 27807067 |
| PMC | PMC5219918 |
| MeSH | Animals Cell Line, Tumor Clathrin / genetics Endocytosis / genetics Endosomes / metabolism Endosomes / pathology Glioma / genetics Glioma / metabolism* Glioma / pathology Heparan Sulfate Proteoglycans / genetics Heparan Sulfate Proteoglycans / metabolism* Humans Proteomics Rats Transport Vesicles / metabolism* Transport Vesicles / pathology rab GTP-Binding Proteins / biosynthesis* rab GTP-Binding Proteins / genetics |
| IF | 3.147 |
| Times Cited | 3 |
| Resource | |
| Human and Animal Cells | C6(RCB2854) |