Reference - Detail
|Author||Kumazoe M, Takai M, Bae J, Hiroi S, Huang Y, Takamatsu K, Won Y, Yamashita M, Hidaka S, Yamashita S, Yamada S, Murata M, Tsukamoto S, Tachibana H.|
|Title||FOXO3 is essential for CD44 expression in pancreatic cancer cells.|
Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal types of cancer and the 5-year survival rate is only 5%. Several studies have suggested that cancer stem cells (CSCs) are thought to be involved in recurrence and metastasis and so it is essential to establish an approach targeting CSCs. Here we have demonstrated that cyclic guanosine monophosphate (cGMP) suppressed CD44 expression and the properties of CSCs in PDAC. Microarray analysis suggested that cGMP inhibited Forkhead box O3 (FOXO3), which is known as a tumor suppressor. Surprisingly, our data demonstrated that FOXO3 is essential for CD44 expression and the properties of CSCs. Our data also indicated that patients with high FOXO3 activation signatures had poor prognoses. This evidence suggested that cGMP induction and FOXO3 inhibition could be ideal candidates for pancreatic CSC.
|MeSH||Adenocarcinoma / genetics* Adenocarcinoma / pathology Animals Biomarkers, Tumor / biosynthesis Biomarkers, Tumor / genetics* Carcinoma, Pancreatic Ductal / genetics* Carcinoma, Pancreatic Ductal / pathology Cell Line, Tumor Cyclic GMP / metabolism Forkhead Box Protein O3 / biosynthesis Forkhead Box Protein O3 / genetics* Gene Expression Regulation, Neoplastic Humans Hyaluronan Receptors / biosynthesis Hyaluronan Receptors / genetics* Mice Microarray Analysis Neoplasm Metastasis Neoplastic Stem Cells / metabolism Neoplastic Stem Cells / pathology Prognosis Xenograft Model Antitumor Assays|
|Human and Animal Cells||PANC-1(RCB2095)|