論文 - 詳細
| RRC ID | 52090 |
|---|---|
| 著者 | Hang Q, Isaji T, Hou S, Wang Y, Fukuda T, Gu J. |
| タイトル | A Key Regulator of Cell Adhesion: Identification and Characterization of Important N-Glycosylation Sites on Integrin α5 for Cell Migration. |
| ジャーナル | Mol Cell Biol |
| Abstract |
The N-glycosylation of integrin α5β1 is thought to control many fundamental aspects of cell behavior, including cell adhesion and migration. However, the mechanism of how N-glycans function remains largely obscure. Here, we used a loss-of-function approach. Wild-type (WT) integrin α5 and N-glycosylation mutant S3-5 (sites 3 to 5) integrin α5, which contains fewer N-glycans, were stably reconstituted in α5 knockout cancer cells. We found that the migration ability of S3-5 cells was decreased in comparison with that of the WT. Interestingly, the levels of phosphorylated focal adhesion kinase and actin stress fiber formation were greatly enhanced in the S3-5 mutant. In a mechanistic manner, the internalization of active but not total integrin α5β1 was inhibited in S3-5 cells, which is a process that is related to the enhanced expression of active integrin α5β1 on the cell surface. Importantly, restoration of N-glycosylation on the β-propeller domain of α5 reinstated the cell migration ability, active α5β1 expression, and internalization. Moreover, these N-glycans are critical for α5-syndecan-4 complex formation. These findings indicate that N-glycosylation on the β-propeller domain functions as a molecular switch to control the dynamics of α5β1 on the cell surface that in turn is required for optimum adhesion for cell migration. |
| 巻・号 | 37(9) |
| 公開日 | 2017-5-1 |
| DOI | 10.1128/MCB.00558-16 |
| PII | MCB.00558-16 |
| PMID | 28167607 |
| PMC | PMC5394273 |
| MeSH | Cell Adhesion / physiology* Cell Line, Tumor Cell Membrane / metabolism* Cell Movement / physiology* Focal Adhesion Kinase 1 / metabolism Glycosylation HEK293 Cells HeLa Cells Humans Integrin alpha5beta1 / metabolism* Multiprotein Complexes / metabolism Phosphorylation Protein Structure, Tertiary Syndecan-4 / metabolism |
| IF | 3.611 |
| 引用数 | 17 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) HeLa |