論文 - 詳細
| RRC ID | 52111 |
|---|---|
| 著者 | Tsukune N, Naito M, Kubota T, Ozawa Y, Nagao M, Ohashi A, Sato S, Takahashi T. |
| タイトル | Lamin A overexpression promotes osteoblast differentiation and calcification in the MC3T3-E1 preosteoblastic cell line. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Lamin A/C is a component of the nuclear lamina, which is involved in cellular proliferation and differentiation. However, the mechanism by which lamin A regulates osteoblast differentiation is not well understood. In this study, we investigated lamin A/C expression during osteoblast differentiation in a preosteoblastic cell line, MC3T3-E1. Real-time PCR analysis showed that lamin A/C mRNA expression was upregulated during BMP-2 induced osteoblast differentiation. Treatment with the estrogen receptor antagonist, fulvestrant, inhibited osteoblast differentiation and the upregulation of lamin A/C mRNA and protein expressions in the presence of BMP-2. These results clearly demonstrated that lamin A/C expression correlates with osteoblast differentiation. To determine the roles of lamin A expression in osteoblast differentiation, MC3T3-E1 cells were transfected with a vector overexpressing lamin A. Results showed that lamin A overexpression promoted osteoblast differentiation and calcification by inducing the expression of alkaline phosphatase, type 1 collagen, BSP, osteocalcin, and DMP-1 in the presence of BMP-2. Furthermore, lamin A overexpression partially restored osteoblastic capacity in the presence of fulvestrant by increasing the expression of BSP, osteocalcin, and DMP-1. These results suggest that lamin A plays important roles in maintaining the osteoblast differentiation and function. |
| 巻・号 | 488(4) |
| ページ | 664-670 |
| 公開日 | 2017-7-8 |
| DOI | 10.1016/j.bbrc.2017.02.110 |
| PII | S0006-291X(17)30401-1 |
| PMID | 28237702 |
| MeSH | Animals Calcification, Physiologic* Cell Differentiation* Cells, Cultured Lamin Type A / genetics* Lamin Type A / metabolism Mice Mice, Inbred C57BL Osteoblasts / cytology* Osteoblasts / metabolism* |
| IF | 2.985 |
| 引用数 | 13 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |