| RRC ID |
52157
|
| 著者 |
Yang L, Huang S, Ma H, Wu X, Feng F.
|
| タイトル |
MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer.
|
| ジャーナル |
Tumour Biol
|
| Abstract |
We intended to investigate the functional role and clinical relevance of microRNA-125b in human gallbladder cancer. Quantitative real-time polymerase chain reaction was used to examine microRNA-125b expression in gallbladder cancer cell lines, and 79 pairs of gallbladder cancer and normal gallbladder clinical tissues. Clinical correlations between tumorous microRNA-125b expression and gallbladder cancer patients' clinicopathological variances or overall survivals were statistically analyzed. In gallbladder cancer cell lines, TYGBK-8 and G-415 cells, microRNA-125b was upregulated to examine its regulatory effect on gallbladder cancer proliferation and migration in vitro. MicroRNA-125b was significantly downregulated in gallbladder cancer cell lines and human gallbladder cancer tumors. MicroRNA-125b in gallbladder cancer was significantly correlated with patients' clinical stage, tumor differentiation, lymph metastasis, and tumor invasion. Low tumorous microRNA-125b expression was also found to be associated with poor overall survivals among gallbladder cancer patients. In vitro studies demonstrated that microRNA-125b upregulation significantly suppressed proliferation and migration in TYGBK-8 and G-415 cells. Tumorous microRNA-125b is an independent prognostic biomarker for patients with gallbladder cancer and possibly acts as a tumor suppressor in gallbladder cancer.
|
| 巻・号 |
39(3)
|
| ページ |
1010428317692249
|
| 公開日 |
2017-3-1
|
| DOI |
10.1177/1010428317692249
|
| PMID |
28347246
|
| MeSH |
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics
Cholecystectomy
Down-Regulation
Gallbladder Neoplasms / genetics*
Gallbladder Neoplasms / metabolism
Gallbladder Neoplasms / pathology
Gallbladder Neoplasms / surgery
Humans
Kaplan-Meier Estimate
MicroRNAs / biosynthesis*
MicroRNAs / genetics
Predictive Value of Tests
Prognosis
Real-Time Polymerase Chain Reaction
|
| IF |
3.65
|
| 引用数 |
3
|
| リソース情報 |
| ヒト・動物細胞 |
G-415(RCB2640)
TGBC1TKB(RCB1129)
TGBC2TKB(RCB1130)
TGBC14TKB(RCB1186)
TGBC24TKB(RCB1196) |
