RRC ID 52157
Author Yang L, Huang S, Ma H, Wu X, Feng F.
Title MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer.
Journal Tumour Biol.
Abstract We intended to investigate the functional role and clinical relevance of microRNA-125b in human gallbladder cancer. Quantitative real-time polymerase chain reaction was used to examine microRNA-125b expression in gallbladder cancer cell lines, and 79 pairs of gallbladder cancer and normal gallbladder clinical tissues. Clinical correlations between tumorous microRNA-125b expression and gallbladder cancer patients' clinicopathological variances or overall survivals were statistically analyzed. In gallbladder cancer cell lines, TYGBK-8 and G-415 cells, microRNA-125b was upregulated to examine its regulatory effect on gallbladder cancer proliferation and migration in vitro. MicroRNA-125b was significantly downregulated in gallbladder cancer cell lines and human gallbladder cancer tumors. MicroRNA-125b in gallbladder cancer was significantly correlated with patients' clinical stage, tumor differentiation, lymph metastasis, and tumor invasion. Low tumorous microRNA-125b expression was also found to be associated with poor overall survivals among gallbladder cancer patients. In vitro studies demonstrated that microRNA-125b upregulation significantly suppressed proliferation and migration in TYGBK-8 and G-415 cells. Tumorous microRNA-125b is an independent prognostic biomarker for patients with gallbladder cancer and possibly acts as a tumor suppressor in gallbladder cancer.
Volume 39(3)
Pages 1010428317692249
Published 2017-3
DOI 10.1177/1010428317692249
PMID 28347246
MeSH Cell Line, Tumor Cell Movement / genetics Cell Proliferation / genetics Cholecystectomy Down-Regulation Gallbladder Neoplasms / genetics* Gallbladder Neoplasms / metabolism Gallbladder Neoplasms / pathology Gallbladder Neoplasms / surgery Humans Kaplan-Meier Estimate MicroRNAs / biosynthesis* MicroRNAs / genetics Predictive Value of Tests Prognosis Real-Time Polymerase Chain Reaction
Human and Animal Cells G-415(RCB2640) TGBC1TKB(RCB1129) TGBC2TKB(RCB1130) TGBC14TKB(RCB1186) TGBC24TKB(RCB1196)