Reference - Detail
RRC ID | 52175 |
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Author | Okuda T. |
Title | PUGNAc treatment provokes globotetraosylceramide accumulation in human umbilical vein endothelial cells. |
Journal | Biochem Biophys Res Commun |
Abstract |
PUGNAc is a well-investigated inhibitor for protein-O-GlcNAcase, whereas recent investigations showed that PUGNAc had a broad range as inhibitor for cellular β-hexosaminidases. Here we report that PUGNAc treatment provokes globotetraosylceramide (Gb4Cer) accumulation in human umbilical vein endothelial cells (HUVEC). HPLC analysis and a quantitative ELISA using newly developed anti-Gb4Cer monoclonal antibody revealed that PUGNAc treatment specifically increased the expression of Gb4Cer among glycosphingolipids expressed in HUVEC. Although the effect was weaker than PUGNAc, an O-GlcNAcase selective inhibitor (Thiamet-G) treatment also increased Gb4Cer levels in HUVEC. Furthermore, both of PUGNAc and Thiamet-G treatment up-regulated the expression levels of α-1,4-galactosyltransferase/Gb3Cer synthase gene which encodes a key enzyme in Gb4Cer synthesis. These results indicate that protein-O-GlcNAcylation can regulate the expression levels of cellular Gb4Cer. |
Volume | 487(1) |
Pages | 76-82 |
Published | 2017-5-20 |
DOI | 10.1016/j.bbrc.2017.04.019 |
PII | S0006-291X(17)30675-7 |
PMID | 28392398 |
MeSH | Acetylglucosamine / administration & dosage Acetylglucosamine / analogs & derivatives* Animals Cells, Cultured Dose-Response Relationship, Drug Endothelial Cells / cytology Endothelial Cells / drug effects* Endothelial Cells / metabolism* Globosides / biosynthesis* Humans Mice Mice, Inbred C3H Oximes / administration & dosage* Phenylcarbamates / administration & dosage* Umbilical Veins / cytology* Umbilical Veins / drug effects Umbilical Veins / metabolism* Up-Regulation / drug effects Up-Regulation / genetics |
IF | 2.985 |
Times Cited | 8 |
Resource | |
Human and Animal Cells | Sp2/0-Ag14(RCB0209) |