| RRC ID |
52396
|
| 著者 |
Saiki A, Motoyoshi M, Motozawa K, Okamura T, Ueki K, Shimizu N, Asano M.
|
| タイトル |
EMMPRIN Inhibits bFGF-Induced IL-6 Secretion in an Osteoblastic Cell Line, MC3T3-E1.
|
| ジャーナル |
Int J Med Sci
|
| Abstract |
Background: Electrolytically-generated acid functional water (FW) is obtained by electrolyzing low concentrations of saline. Although it has been widely used in clinical practice with various purposes, the underlying mechanisms of action involved have not been fully elucidated so far. We used the human cervical cancer-derived fibroblastic cell line (HeLa), to examine the cytokine secretion profile following FW treatment in the present study. Results: FW stimulation significantly induced the secretion of basic fibroblast growth factor (bFGF) and extracellular matrix metalloproteinase inducer (EMMPRIN). The effect of both factors on osteoblast-like MC3T3-E1 cells was further examined by stimulating the cells with the conditioned medium of FW-stimulated HeLa cells. However, the conditioned medium failed to induce IL-6 secretion. The MC3T3-E1 cells were further stimulated with recombinant bFGF or EMMPRIN or a combination of both factors. Intriguingly, bFGF-stimulated IL-6 induction was totally inhibited by EMMPRIN. Pretreatment with the specific inhibitor of nuclear factor-kappa B (NF-κB) drastically inhibited IL-6 secretion indicating that bFGF-induced IL-6 expression was dependent on NF-κB activation. The phosphorylation status of NF-κB p65 subunit was further examined. The results indicated that EMMPRIN inhibited bFGF-induced NF-κB p65 phosphorylation. Conclusions: These findings suggest that bFGF can induce IL-6 secretion in MC3T3-E1 cells through NF-κB activation. As EMMPRIN inhibited bFGF-induced IL-6 secretion by reducing the p65 subunit phosphorylation, it might be concluded that bFGF and EMMPRIN crosstalk in their respective signaling pathways.
|
| 巻・号 |
14(12)
|
| ページ |
1173-1180
|
| 公開日 |
2017-9-19
|
| DOI |
10.7150/ijms.20387
|
| PII |
ijmsv14p1173
|
| PMID |
29104472
|
| PMC |
PMC5666549
|
| MeSH |
Animals
Basigin / metabolism*
Cell Culture Techniques / methods
Culture Media, Conditioned
Disinfectants / chemistry
Disinfectants / pharmacology
Electrolysis
Fibroblast Growth Factor 2 / metabolism*
HeLa Cells
Humans
Interleukin-6 / metabolism*
Mice
Osteoblasts
Phosphorylation
Recombinant Proteins / metabolism
Signal Transduction / drug effects
Transcription Factor RelA / antagonists & inhibitors
Transcription Factor RelA / metabolism*
Water / chemistry
Water / pharmacology
|
| IF |
2.333
|
| 引用数 |
4
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
