RRC ID 52424
Author Elfeky M, Yoneshiro T, Okamatsu-Ogura Y, Kimura K.
Title Adiponectin suppression of late inflammatory mediator, HMGB1-induced cytokine expression in RAW264 macrophage cells.
Journal J Biochem
Abstract High-mobility group protein B1 (HMGB1) is a late inflammatory mediator released from inflammatory cells when stimulated, resulting in exaggerating septic symptoms. We recently demonstrated that full-length adiponectin, a potent anti-inflammatory adipokine, inhibits lipopolysaccharide-induced HMGB1 release. However, the effects of adiponectin on HMGB1-induced exaggerating signals currently remain unknown. This study aimed to investigate the effects of adiponectin on the pro-inflammatory function of HMGB1 in RAW264 macrophage cells. The treatment of RAW264 cells with HMGB1 significantly up-regulated the mRNA expression of tumour necrosis factor-α, interleukin-1β and C-X-C motif chemokine 10. HMGB1-induced cytokine expression was markedly suppressed by a toll-like receptor 4 (TLR4) antagonist and slightly suppressed by an antagonist of the receptor for advanced glycation end products. A prior treatment with full-length or globular adiponectin dose-dependently suppressed all types of HMGB1-induced cytokine expression, and this suppression was abolished by compound C, an AMPK inhibitor, but not by the haem oxygenase (HO)-1 inhibitor, zinc protoporphyrin IX. Both forms of adiponectin also reduced the mRNA expression of TLR4. These results suggest that full-length and globular adiponectin suppress HMGB1-induced cytokine expression through an AMPK-mediated HO-1-independent pathway.
Volume 163(2)
Pages 143-153
Published 2018-2-1
DOI 10.1093/jb/mvx069
PII 4557162
PMID 29048484
MeSH Adiponectin / pharmacology* Animals Cells, Cultured Cytokines / biosynthesis* Dose-Response Relationship, Drug HMGB1 Protein / antagonists & inhibitors* HMGB1 Protein / isolation & purification HMGB1 Protein / metabolism Inflammation Mediators / antagonists & inhibitors* Inflammation Mediators / metabolism Macrophages / drug effects* Macrophages / metabolism Mice RAW 264.7 Cells Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism Structure-Activity Relationship
IF 2.23
Times Cited 2
Human and Animal Cells RAW 264(RCB0535)