論文 - 詳細
| RRC ID | 52424 |
|---|---|
| 著者 | Elfeky M, Yoneshiro T, Okamatsu-Ogura Y, Kimura K. |
| タイトル | Adiponectin suppression of late inflammatory mediator, HMGB1-induced cytokine expression in RAW264 macrophage cells. |
| ジャーナル | J Biochem |
| Abstract |
High-mobility group protein B1 (HMGB1) is a late inflammatory mediator released from inflammatory cells when stimulated, resulting in exaggerating septic symptoms. We recently demonstrated that full-length adiponectin, a potent anti-inflammatory adipokine, inhibits lipopolysaccharide-induced HMGB1 release. However, the effects of adiponectin on HMGB1-induced exaggerating signals currently remain unknown. This study aimed to investigate the effects of adiponectin on the pro-inflammatory function of HMGB1 in RAW264 macrophage cells. The treatment of RAW264 cells with HMGB1 significantly up-regulated the mRNA expression of tumour necrosis factor-α, interleukin-1β and C-X-C motif chemokine 10. HMGB1-induced cytokine expression was markedly suppressed by a toll-like receptor 4 (TLR4) antagonist and slightly suppressed by an antagonist of the receptor for advanced glycation end products. A prior treatment with full-length or globular adiponectin dose-dependently suppressed all types of HMGB1-induced cytokine expression, and this suppression was abolished by compound C, an AMPK inhibitor, but not by the haem oxygenase (HO)-1 inhibitor, zinc protoporphyrin IX. Both forms of adiponectin also reduced the mRNA expression of TLR4. These results suggest that full-length and globular adiponectin suppress HMGB1-induced cytokine expression through an AMPK-mediated HO-1-independent pathway. |
| 巻・号 | 163(2) |
| ページ | 143-153 |
| 公開日 | 2018-2-1 |
| DOI | 10.1093/jb/mvx069 |
| PII | 4557162 |
| PMID | 29048484 |
| MeSH | Adiponectin / pharmacology* Animals Cells, Cultured Cytokines / biosynthesis* Dose-Response Relationship, Drug HMGB1 Protein / antagonists & inhibitors* HMGB1 Protein / isolation & purification HMGB1 Protein / metabolism Inflammation Mediators / antagonists & inhibitors* Inflammation Mediators / metabolism Macrophages / drug effects* Macrophages / metabolism Mice RAW 264.7 Cells Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism Structure-Activity Relationship |
| IF | 2.476 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |