RRC ID 52462
著者 Yoshida K, Teramachi J, Uchibe K, Ikegame M, Qiu L, Yang D, Okamura H.
タイトル Reduction of protein phosphatase 2A Cα promotes in vivo bone formation and adipocyte differentiation.
ジャーナル Mol Cell Endocrinol
Abstract Serine/threonine protein phosphatase 2A (PP2A) regulates diverse physiological processes such as cell cycle, growth, apoptosis, and signal transduction. Previously, we demonstrated that silencing of the α-isoform of PP2A catalytic subunit (PP2A Cα) in osteoblasts accelerated osteoblast differentiation, whereas its overexpression suppressed differentiation. In this study, we examined the role of PP2A Cα in in vivo bone formation by generating transgenic mice (PP2A-Tg), in which the dominant negative form of PP2A Cα was specifically expressed in osteoblasts. PP2A-Tg mice exhibited an increase in body weight, cortical bone mineral density, and cortical bone thickness. Interestingly, they also displayed higher amounts of adipose tissue in the bone marrow of tibiae. The co-culture study showed that PP2A Cα-knockdown osteoblasts stimulated adipocyte differentiation from undifferentiated mesenchymal cells via upregulation of the adipocyte marker genes, such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα). These results indicated that the reduction of PP2A Cα levels in osteoblasts promoted bone formation in vivo. Additionally, PP2A Cα in osteoblasts was also potentially involved in controlling adipocyte differentiation through a paracrine mechanism.
巻・号 470
ページ 251-258
公開日 2018-7-15
DOI 10.1016/j.mce.2017.11.005
PII S0303-7207(17)30570-1
PMID 29128580
MeSH Adipocytes / cytology* Adipocytes / metabolism* Adipogenesis Animals Biomarkers / metabolism Bone Density Cell Differentiation* Cell Line Collagen Type I / metabolism Cortical Bone / anatomy & histology Gene Knockdown Techniques Mice, Transgenic Osteoblasts / metabolism Osteogenesis* Protein Phosphatase 2 / metabolism* Up-Regulation
IF 3.871
引用数 3
リソース情報
ヒト・動物細胞 10T1/2(RCB0247)