Reference - Detail
| RRC ID | 52627 |
|---|---|
| Author | Yamada N, Kato-Kogoe N, Yamanegi K, Nishiura H, Fujihara Y, Fukunishi S, Okamura H, Terada N, Nakasho K. |
| Title | Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Hypoxia down-regulates the expression of cell surface major histocompatibility class I-related chain molecule A (MICA) without increasing its shedding. Recently, the inhibition of N-linked glycosylation was also shown to reduce the cell-surface expression of MICA. We investigated the participation of asparagine (Asn)-linked glycosylation in hypoxia-induced down-regulation of cell-surface MICA using osteosarcoma cells. MATERIALS AND METHODS:The cell-surface expression and Asn-N-glycosylation of MICA were estimated by flow cytometry, and western blot analyses, respectively. RESULTS:Hypoxia reduced the expression of N-linked glycosylated MICA, as well as the ratio of N-linked glycosylated to non-glycosylated MICA. 2-Deoxy-D-glucose, which inhibits N-linked glycosylation, reduced the cell-surface expression of MICA under normoxia, while D-Mannose increased N-glycosylated MICA, increasing cell-surface MICA under hypoxia. Cells transfected with wild-type MICA expression vector expressed cell surface MICA more than those transfected with mutant MICA expression vectors designed for abrogation of N-linked glycosylation. CONCLUSION:The inhibition of Asn-N-linked glycosylation participates in hypoxia-induced down-regulation of cell-surface expression of MICA. |
| Volume | 38(3) |
| Pages | 1353-1359 |
| Published | 2018-3-1 |
| DOI | 10.21873/anticanres.12358 |
| PII | 38/3/1353 |
| PMID | 29491059 |
| MeSH | Amino Acid Sequence Asparagine / genetics* Asparagine / metabolism Cell Hypoxia Cell Line, Tumor Cell Membrane / genetics Cell Membrane / metabolism Deoxyglucose / pharmacology Down-Regulation* Gene Expression Regulation, Neoplastic* Glycosylation / drug effects Histocompatibility Antigens Class I / genetics* Histocompatibility Antigens Class I / metabolism Humans Osteosarcoma / genetics Osteosarcoma / metabolism Osteosarcoma / pathology |
| IF | 1.994 |
| Times Cited | 3 |
| Resource | |
| Human and Animal Cells | HOS(RCB0992) |