Reference - Detail
RRC ID | 52628 |
---|---|
Author | Yajima Y, Lee CN, Yamada M, Utoh R, Seki M. |
Title | Development of a perfusable 3D liver cell cultivation system via bundling-up assembly of cell-laden microfibers. |
Journal | J Biosci Bioeng |
Abstract |
Although the reconstruction of functional 3D liver tissue models in vitro presents numerous challenges, it is in great demand for drug development, regenerative medicine, and physiological studies. Here we propose a new approach to perform perfusion cultivation of liver cells by assembling cell-laden hydrogel microfibers. HepG2 cells were densely packed into the core of sandwich-type anisotropic microfibers, which were produced using microfluidic devices. The obtained microfibers were bundled up and packed into a perfusion chamber, and perfusion cultivation was performed. We evaluated cell viability and functions, and also monitored the oxygen consumption. Furthermore, fibers covered with vascular endothelial cells were united during the perfusion culture, to form vascular network-like conduits between fibers. The presented technique can structurally mimic the hepatic lobule in vivo and could prove to be a useful model for various biomedical research applications. |
Volume | 126(1) |
Pages | 111-118 |
Published | 2018-7-1 |
DOI | 10.1016/j.jbiosc.2018.01.022 |
PII | S1389-1723(17)31108-8 |
PMID | 29502942 |
MeSH | Animals Cattle Cell Culture Techniques* / instrumentation Cell Culture Techniques* / methods Cells, Cultured Coculture Techniques Endothelial Cells / cytology Hep G2 Cells Hepatocytes / cytology* Humans Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry Lab-On-A-Chip Devices Liver / cytology* Microtechnology / instrumentation Microvessels / cytology Perfusion Regenerative Medicine / instrumentation Regenerative Medicine / methods Tissue Engineering / instrumentation* Tissue Engineering / methods* Tissue Scaffolds |
IF | 2.366 |
Times Cited | 13 |
Resource | |
Human and Animal Cells | Hep G2(RCB1886) |