論文 - 詳細
| RRC ID | 52648 |
|---|---|
| 著者 | Kobayashi N, Kawasaki-Nishi S, Otsuka M, Hisano Y, Yamaguchi A, Nishi T. |
| タイトル | MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells. |
| ジャーナル | Sci Rep |
| Abstract |
Sphingosine 1-phosphate (S1P) is an intercellular signaling molecule present in blood. Erythrocytes have a central role in maintaining the S1P concentration in the blood stream. We previously demonstrated that S1P is exported from erythrocytes by a glyburide-sensitive S1P transporter. However, the gene encoding the S1P transporter in erythrocytes is unknown. In this study, we found that the mouse erythroid cell line, MEDEP-E14, has S1P export activity and exhibits properties that are consistent with those of erythrocytes. Using microarray analysis of MEDEP-E14 cells and its parental cell line, E14TG2a, we identified several candidate genes for S1P export activity. Of those genes, only one gene, Mfsd2b, showed S1P transport activity. The properties of S1P release by MFSD2B were similar to those in erythrocytes. Moreover, knockout of MFSD2B in MEDEP-E14 cells decreased S1P export from the cells. These results strongly suggest that MFSD2B is a novel S1P transporter in erythroid cells. |
| 巻・号 | 8(1) |
| ページ | 4969 |
| 公開日 | 2018-3-21 |
| DOI | 10.1038/s41598-018-23300-x |
| PII | 10.1038/s41598-018-23300-x |
| PMID | 29563527 |
| PMC | PMC5862976 |
| MeSH | Animals CHO Cells Cell Line Cricetulus Erythroid Cells / metabolism* Gene Knockout Techniques Lysophospholipids / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Microarray Analysis Phosphotransferases (Alcohol Group Acceptor) / genetics Phosphotransferases (Alcohol Group Acceptor) / metabolism Recombinant Proteins / genetics Recombinant Proteins / metabolism Sphingosine / analogs & derivatives* Sphingosine / metabolism |
| IF | 3.998 |
| 引用数 | 15 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | E14tg2a(AES0135) MEDEP-E14(RCB2660) |