RRC ID 52648
Author Kobayashi N, Kawasaki-Nishi S, Otsuka M, Hisano Y, Yamaguchi A, Nishi T.
Title MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells.
Journal Sci Rep
Abstract Sphingosine 1-phosphate (S1P) is an intercellular signaling molecule present in blood. Erythrocytes have a central role in maintaining the S1P concentration in the blood stream. We previously demonstrated that S1P is exported from erythrocytes by a glyburide-sensitive S1P transporter. However, the gene encoding the S1P transporter in erythrocytes is unknown. In this study, we found that the mouse erythroid cell line, MEDEP-E14, has S1P export activity and exhibits properties that are consistent with those of erythrocytes. Using microarray analysis of MEDEP-E14 cells and its parental cell line, E14TG2a, we identified several candidate genes for S1P export activity. Of those genes, only one gene, Mfsd2b, showed S1P transport activity. The properties of S1P release by MFSD2B were similar to those in erythrocytes. Moreover, knockout of MFSD2B in MEDEP-E14 cells decreased S1P export from the cells. These results strongly suggest that MFSD2B is a novel S1P transporter in erythroid cells.
Volume 8(1)
Pages 4969
Published 2018-3-21
DOI 10.1038/s41598-018-23300-x
PII 10.1038/s41598-018-23300-x
PMID 29563527
PMC PMC5862976
MeSH Animals CHO Cells Cell Line Cricetulus Erythroid Cells / metabolism* Gene Knockout Techniques Lysophospholipids / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Microarray Analysis Phosphotransferases (Alcohol Group Acceptor) / genetics Phosphotransferases (Alcohol Group Acceptor) / metabolism Recombinant Proteins / genetics Recombinant Proteins / metabolism Sphingosine / analogs & derivatives* Sphingosine / metabolism
IF 3.998
Times Cited 15
Human and Animal Cells E14tg2a(AES0135) MEDEP-E14(RCB2660)