RRC ID 52673
Author Yamashita W, Takahashi M, Kikkawa T, Gotoh H, Osumi N, Ono K, Nomura T.
Title Conserved and divergent functions of Pax6 underlie species-specific neurogenic patterns in the developing amniote brain.
Journal Development
Abstract The evolution of unique organ structures is associated with changes in conserved developmental programs. However, characterizing the functional conservation and variation of homologous transcription factors (TFs) that dictate species-specific cellular dynamics has remained elusive. Here, we dissect shared and divergent functions of Pax6 during amniote brain development. Comparative functional analyses revealed that the neurogenic function of Pax6 is highly conserved in the developing mouse and chick pallium, whereas stage-specific binary functions of Pax6 in neurogenesis are unique to mouse neuronal progenitors, consistent with Pax6-dependent temporal regulation of Notch signaling. Furthermore, we identified that Pax6-dependent enhancer activity of Dbx1 is extensively conserved between mammals and chick, although Dbx1 expression in the developing pallium is highly divergent in these species. Our results suggest that spatiotemporal changes in Pax6-dependent regulatory programs contributed to species-specific neurogenic patterns in mammalian and avian lineages, which underlie the morphological divergence of the amniote pallial architectures.
Volume 145(8)
Published 2018-4-16
DOI 10.1242/dev.159764
PII 145/8/dev159764
PMID 29661783
PMC PMC5964652
MeSH Animals Animals, Genetically Modified Avian Proteins / genetics Avian Proteins / physiology* Brain / embryology* Brain / physiology* Chick Embryo Enhancer Elements, Genetic Evolution, Molecular Female Gene Deletion Gene Expression Regulation, Developmental Homeodomain Proteins / genetics Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Knockout Mice, Transgenic Neurogenesis / genetics Neurogenesis / physiology PAX6 Transcription Factor / deficiency PAX6 Transcription Factor / genetics PAX6 Transcription Factor / physiology* Pregnancy Receptors, Notch / genetics Receptors, Notch / physiology Signal Transduction Species Specificity
IF 5.763
Times Cited 6
Resource
Human and Animal Cells 293T(RCB2202)