RRC ID 52777
Author Mun T, Małolepszy A, Sandal N, Stougaard J, Andersen SU.
Title User Guide for the LORE1 Insertion Mutant Resource.
Journal Methods Mol Biol
Abstract Lotus japonicus is a model legume used in the study of plant-microbe interactions, especially in the field of biological nitrogen fixation due to its ability to enter into a symbiotic relationship with a soil bacterium, Mesorhizobium loti. The LORE1 mutant population is a valuable resource for reverse genetics in L. japonicus due to its non-transgenic nature, high tagging efficiency, and low copy count. Here, we outline a workflow for identifying, ordering, and establishing homozygous LORE1 mutant lines for a gene of interest, LjFls2, including protocols for growth and genotyping of a segregating LORE1 population.
Volume 1610
Pages 13-23
Published 2017-1-1
DOI 10.1007/978-1-4939-7003-2_2
PMID 28439854
MeSH Fabaceae / genetics Fabaceae / microbiology Genotype Lotus / genetics* Lotus / microbiology Mutagenesis, Insertional / genetics* Mutation / genetics Plant Roots / genetics Plant Roots / microbiology Symbiosis / genetics Symbiosis / physiology
Times Cited 0
Resource
Lotus / Glycine Gifu B-129