RRC ID 52813
Author Yamaguchi A, Matsumura R, Matsuzaki T, Nakamura W, Node K, Akashi M.
Title A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans.
Journal Sci Rep
Abstract Almost all organisms maintain a circadian clock from birth to death to synchronize their own physiology and behavior with the earth's rotation. Because the in vivo evaluation of human circadian characteristics is labor-intensive, in vitro or ex vivo approaches could provide advantages. In this study, to enable the simple and non-invasive evaluation of autonomous circadian oscillation, we established a method for monitoring clock gene expression by performing ex vivo culture of whole hair root tissue. This method is extremely simple and imposes little burden on subjects. Results obtained using Cryptochrome-deficient mice support that circadian period length in hair tissue correlates with intrinsic period length observed in physiology and behavior. We then applied this method to old-old subjects with severe dementia, who showed abnormal circadian behavior, and found that their peripheral clocks autonomously oscillated in a manner similar to those of healthy or younger subjects, indicating that the effect of cellular senescence on the autonomous clock oscillator is limited at least in some cell types. Although further validation may be required, the hair tissue-based culture assay would be a tool to investigate intrinsic circadian characteristics in humans.
Volume 7(1)
Pages 6824
Published 2017-7-28
DOI 10.1038/s41598-017-07268-8
PII 10.1038/s41598-017-07268-8
PMID 28755004
PMC PMC5533706
MeSH Aged, 80 and over Aging / metabolism Animals Cells, Cultured Chronobiology Disorders / metabolism* Circadian Rhythm* Cryptochromes / genetics Cryptochromes / metabolism Female Hair Follicle / cytology Hair Follicle / metabolism* Humans Male Mice
IF 3.998
Times Cited 4
Resource
DNA material mouse Period2 (-2.8 - +0.1 kb)_luciferase/pENTR-1A (RDB15083) human Period3 (-3.1 - +0.2 kb)_luciferase/pENTR-1A (RDB15084) human Bmal1 (-1.7 - +0.1 kb)_luciferase/pENTR-1A (RDB15085).