RRC ID 52892
Author Elrouby N, Bonequi MV, Porri A, Coupland G.
Title Identification of Arabidopsis SUMO-interacting proteins that regulate chromatin activity and developmental transitions.
Journal Proc Natl Acad Sci U S A
Abstract Posttranslational modification of proteins by small ubiquitin-like modifier (SUMO) plays essential roles in eukaryotic growth and development. Many covalently modified SUMO targets have been identified; however, the extent and significance of noncovalent interactions of SUMO with cellular proteins is poorly understood. Here, large-scale yeast two-hybrid screens repeatedly identified a surprisingly small number of proteins that interacted with three Arabidopsis SUMO isoforms. These SUMO-interacting proteins are nuclear and fall into two main categories: six histone or DNA methyltransferses or demethylases and six proteins that we show to be the evolutionary and functional homologs of SUMO-targeted ubiquitin ligases (STUbLs). The selectivity of the screen for several methylases and demethylases suggests that SUMO interaction with these proteins has a significant impact on chromatin methylation. Furthermore, the Arabidopsis STUbLs (AT-STUbLs) complemented to varying degrees the growth defects of the Schizosaccharomyces pombe STUbL mutant rfp1/rfp2, and three of them also complemented the genome integrity defects of this mutant, demonstrating that these proteins show STUbL activity. We show that one of the AT-STUbLs least related to the S. pombe protein, AT-STUbL4, has acquired a plant-specific function in the floral transition. It reduces protein levels of CYCLING DOF FACTOR 2, hence increasing transcript levels of CONSTANS and promoting flowering through the photoperiodic pathway.
Volume 110(49)
Pages 19956-61
Published 2013-12-3
DOI 10.1073/pnas.1319985110
PII 1319985110
PMID 24255109
PMC PMC3856783
MeSH Arabidopsis / genetics* Arabidopsis / growth & development* Arabidopsis / metabolism Arabidopsis Proteins / metabolism* Chromatin / metabolism DNA Primers / genetics Gene Library Methylation Nuclear Proteins / metabolism Two-Hybrid System Techniques Ubiquitins / metabolism*
IF 9.58
Times Cited 38
Resource
Arabidopsis / Cultured plant cells, genes pda11253 pda11456