RRC ID 5300
Author Tanasupawat S, Namwong S, Kudo T, Itoh T.
Title Piscibacillus salipiscarius gen. nov., sp. nov., a moderately halophilic bacterium from fermented fish (pla-ra) in Thailand.
Journal Int. J. Syst. Evol. Microbiol.
Abstract A Gram-positive, spore-forming and moderately halophilic bacterium was isolated from fermented fish (pla-ra) in Thailand. Cells of the isolate, RBU1-1(T), were strictly aerobic, motile rods and contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Menaquinone with seven isoprene units (MK-7) was the predominant quinone. This isolate grew at 15-48 degrees C, pH 5-9 and in 2-30 % NaCl (optimally 10-20 %). The major cellular fatty acids were iso-C(15 : 0) and anteiso-C(15 : 0). Polar lipid analysis revealed the presence of phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 36.7 mol%. 16S rRNA gene sequence analysis revealed that strain RBU1-1(T) was a member of the family Bacillaceae, and belonged to a cluster with Filobacillus and Tenuibacillus; strain RBU1-1(T) showed 16S rRNA gene sequence similarities of 96.0-96.9 % to members of these two genera. Strain RBU1-1(T) could also be differentiated from members of the genera Filobacillus and Tenuibacillus based on certain phenotypic characteristics such as cell-wall composition, mode of flagellation and growth pH range. Therefore, strain RBU1-1(T) is considered to represent a novel species in a new genus in the family Bacillaceae, for which the name Piscibacillus salipiscarius gen. nov., sp. nov. is proposed. The type strain of Piscibacillus salipiscarius is RBU1-1(T) (=JCM 13188(T)=PCU 270(T)=TISTR 1571(T)).
Volume 57(Pt 7)
Pages 1413-7
Published 2007-7
DOI 10.1099/ijs.0.64945-0
PII 57/7/1413
PMID 17625167
MeSH Aerobiosis Animals Bacillaceae / chemistry Bacillaceae / classification* Bacillaceae / isolation & purification* Bacillaceae / physiology Bacterial Typing Techniques Base Composition Cell Wall / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Diaminopimelic Acid / analysis Fatty Acids / analysis Fishes / microbiology* Food Microbiology* Genes, rRNA Hydrogen-Ion Concentration Locomotion Microscopy, Electron, Scanning Molecular Sequence Data Peptidoglycan / chemistry Phospholipids / analysis Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / metabolism Spores, Bacterial Temperature Thailand
IF 1.932
Times Cited 30
WOS Category MICROBIOLOGY
Resource
General Microbes JCM12288 JCM13188