| RRC ID |
53110
|
| Author |
Htet AN, Noguchi M, Ninomiya K, Tsuge Y, Kuroda K, Kajita S, Masai E, Katayama Y, Shikinaka K, Otsuka Y, Nakamura M, Honda R, Takahashi K.
|
| Title |
Application of microalgae hydrolysate as a fermentation medium for microbial production of 2-pyrone 4,6-dicarboxylic acid.
|
| Journal |
J Biosci Bioeng
|
| Abstract |
Actual biomass of microalgae was tested as a fermentation substrate for microbial production of 2-pyrone 4,6-dicarboxylic acid (PDC). Acid-hydrolyzed green microalgae Chlorella emersonii (algae hydrolysate) was diluted to adjust the glucose concentration to 2 g/L and supplemented with the nutrients of Luria-Bertani (LB) medium (tryptone 10 g/L and yeast extract 5 g/L). When the algae hydrolysate was used as a fermentation source for recombinant Escherichia coli producing PDC, 0.43 g/L PDC was produced with a yield of 20.1% (mol PDC/mol glucose), whereas 0.19 g/L PDC was produced with a yield of 8.6% when LB medium supplemented with glucose was used. To evaluate the potential of algae hydrolysate alone as a fermentation medium for E. coli growth and PDC production, the nutrients of LB medium were reduced from the algae hydrolysate medium. Interestingly, 0.17 g/L PDC was produced even without additional nutrient, which was comparable to the case using pure glucose medium with nutrients of LB medium. When using a high concentration of hydrolysate without additional nutrients, 1.22 g/L PDC was produced after a 24-h cultivation with the yield of 16.1%. Overall, C. emersonii has high potential as cost-effective fermentation substrate for the microbial production of PDC.
|
| Volume |
125(6)
|
| Pages |
717-722
|
| Published |
2018-6-1
|
| DOI |
10.1016/j.jbiosc.2017.12.026
|
| PII |
S1389-1723(17)31105-2
|
| PMID |
29395960
|
| MeSH |
Biomass
Carboxylic Ester Hydrolases / metabolism
Chlorella / enzymology
Chlorella / growth & development
Chlorella / metabolism*
Escherichia coli / genetics
Escherichia coli / metabolism*
Fermentation*
Glucose / metabolism
Hydrolysis
Microalgae / enzymology
Microalgae / growth & development
Microalgae / metabolism*
Organisms, Genetically Modified
Pyrones / metabolism*
|
| IF |
2.366
|
| Times Cited |
6
|
| Resource |
| Algae |
NIES-2151 |
