論文 - 詳細
| RRC ID | 53186 |
|---|---|
| 著者 | Nagaki K, Yamaji N, Murata M. |
| タイトル | ePro-ClearSee: a simple immunohistochemical method that does not require sectioning of plant samples. |
| ジャーナル | Sci Rep |
| Abstract |
Investigations into the epigenetic status of individual cells within tissues can produce both epigenetic data for different cell types and positional information of the cells. Thus, these investigations are important for understanding the intra- and inter-cellular control systems of developmental and environmental responses in plants. However, a simple method to detect epigenetic modifications of individual cells in plant tissues is not yet available because detection of the modifications requires immunohistochemistry using specific antibodies. In this study, we developed a simple immunohistochemical method that does not require sectioning to investigate epigenetic modifications. This method uses a clearing system to detect methylated histones, acetylated histones, methylated DNA and/or centromeric histone H3 variants. Analyses of four dicots and five monocots indicated that this method provides a universal technique to investigate epigenetic modifications in diverse plant species. |
| 巻・号 | 7 |
| ページ | 42203 |
| 公開日 | 2017-2-8 |
| DOI | 10.1038/srep42203 |
| PII | srep42203 |
| PMID | 28176832 |
| PMC | PMC5297246 |
| MeSH | Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism Centromere Protein A / genetics Centromere Protein A / metabolism Epigenesis, Genetic* Garlic / genetics Garlic / growth & development Garlic / metabolism Helianthus / genetics Helianthus / growth & development Helianthus / metabolism Histones / genetics Histones / metabolism Hordeum / genetics Hordeum / growth & development Hordeum / metabolism Immunohistochemistry / methods* Oryza / genetics Oryza / growth & development Oryza / metabolism Plant Leaves / genetics Plant Leaves / growth & development Plant Leaves / metabolism Plant Proteins / genetics* Plant Proteins / metabolism Solanum lycopersicum / genetics Solanum lycopersicum / growth & development Solanum lycopersicum / metabolism Tobacco / genetics Tobacco / growth & development Tobacco / metabolism Triticum / genetics Triticum / growth & development Triticum / metabolism Tubulin / genetics Tubulin / metabolism Urea / chemistry* Xylitol / chemistry* Zea mays / genetics Zea mays / growth & development Zea mays / metabolism |
| IF | 3.998 |
| 引用数 | 5 |
| リソース情報 | |
| トマト | TOMJPF00001 |