Reference - Detail
|Author||Ito Y, Hasauda H, Kitajima T, Kiyono T.|
|Title||Ex vivo expansion of human cord blood hematopoietic progenitor cells using glutaraldehyde-fixed human bone marrow stromal cells.|
|Journal||J. Biosci. Bioeng.|
Human stromal cells were immortalized and fixed with glutaraldehyde to support an ex vivo expansion of human cord blood hematopoietic progenitor cells. In addition, this enabled glutaraldehyde-fixed stromal cells to be stored at 4 degrees C. Although freeze-dried glutaraldehyde-fixed stromal cells did not increase the number of the progenitor cells, the percent decrease in the number of CD34(+) cells in the presence of freeze-dried glutaraldehyde-fixed stromal cells was less than that in the absence of the stromal cells. Thus, glutaraldehyde-fixed stromal cells can serve as a stabilizing device for hematopoietic cell expansion.
|MeSH||Antigens, CD34 / analysis Bone Marrow Cells / physiology* Cells, Cultured Fetal Blood / cytology* Glutaral / pharmacology Hematopoiesis* Hematopoietic Stem Cells / cytology* Humans Stromal Cells / physiology|
|WOS Category||FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY|
|Cord blood stem cells for research|