RRC ID 53207
Author Ito Y, Hasauda H, Kitajima T, Kiyono T.
Title Ex vivo expansion of human cord blood hematopoietic progenitor cells using glutaraldehyde-fixed human bone marrow stromal cells.
Journal J. Biosci. Bioeng.
Abstract Human stromal cells were immortalized and fixed with glutaraldehyde to support an ex vivo expansion of human cord blood hematopoietic progenitor cells. In addition, this enabled glutaraldehyde-fixed stromal cells to be stored at 4 degrees C. Although freeze-dried glutaraldehyde-fixed stromal cells did not increase the number of the progenitor cells, the percent decrease in the number of CD34(+) cells in the presence of freeze-dried glutaraldehyde-fixed stromal cells was less than that in the absence of the stromal cells. Thus, glutaraldehyde-fixed stromal cells can serve as a stabilizing device for hematopoietic cell expansion.
Volume 102(5)
Pages 467-9
Published 2006-11
DOI 10.1263/jbb.102.467
PII S1389-1723(06)70693-4
PMID 17189177
MeSH Antigens, CD34 / analysis Bone Marrow Cells / physiology* Cells, Cultured Fetal Blood / cytology* Glutaral / pharmacology Hematopoiesis* Hematopoietic Stem Cells / cytology* Humans Stromal Cells / physiology
IF 2.032
Times Cited 23
Cord blood stem cells for research