論文 - 詳細
| RRC ID | 53248 |
|---|---|
| 著者 | Qadota H, Mayans O, Matsunaga Y, McMurry JL, Wilson KJ, Kwon GE, Stanford R, Deehan K, Tinley TL, Ngwa VM, Benian GM. |
| タイトル | The SH3 domain of UNC-89 (obscurin) interacts with paramyosin, a coiled-coil protein, in Caenorhabditis elegans muscle. |
| ジャーナル | Mol Biol Cell |
| Abstract |
UNC-89 is a giant polypeptide located at the sarcomeric M-line of Caenorhabditis elegans muscle. The human homologue is obscurin. To understand how UNC-89 is localized and functions, we have been identifying its binding partners. Screening a yeast two-hybrid library revealed that UNC-89 interacts with paramyosin. Paramyosin is an invertebrate-specific coiled-coil dimer protein that is homologous to the rod portion of myosin heavy chains and resides in thick filament cores. Minimally, this interaction requires UNC-89's SH3 domain and residues 294-376 of paramyosin and has a KD of ∼1.1 μM. In unc-89 loss-of-function mutants that lack the SH3 domain, paramyosin is found in accumulations. When the SH3 domain is overexpressed, paramyosin is mislocalized. SH3 domains usually interact with a proline-rich consensus sequence, but the region of paramyosin that interacts with UNC-89's SH3 is α-helical and lacks prolines. Homology modeling of UNC-89's SH3 suggests structural features that might be responsible for this interaction. The SH3-binding region of paramyosin contains a "skip residue," which is likely to locally unwind the coiled-coil and perhaps contributes to the binding specificity. |
| 巻・号 | 27(10) |
| ページ | 1606-20 |
| 公開日 | 2016-5-15 |
| DOI | 10.1091/mbc.E15-09-0675 |
| PII | mbc.E15-09-0675 |
| PMID | 27009202 |
| PMC | PMC4865318 |
| MeSH | Actin Cytoskeleton / metabolism Amino Acid Sequence Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Guanine Nucleotide Exchange Factors / metabolism Muscle Proteins / genetics Muscle Proteins / metabolism* Muscles / metabolism Peptides / metabolism Protein Binding Sarcomeres / metabolism Structure-Activity Relationship Tropomyosin / genetics Tropomyosin / metabolism* src Homology Domains |
| IF | 3.791 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm752 |