Reference - Detail
| RRC ID | 53263 |
|---|---|
| Author | Truttmann MC, Cruz VE, Guo X, Engert C, Schwartz TU, Ploegh HL. |
| Title | The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones. |
| Journal | PLoS Genet |
| Abstract |
Protein AMPylation by Fic domain-containing proteins (Fic proteins) is an ancient and conserved post-translational modification of mostly unexplored significance. Here we characterize the Caenorhabditis elegans Fic protein FIC-1 in vitro and in vivo. FIC-1 is an AMPylase that localizes to the nuclear surface and modifies core histones H2 and H3 as well as heat shock protein 70 family members and translation elongation factors. The three-dimensional structure of FIC-1 is similar to that of its human ortholog, HYPE, with 38% sequence identity. We identify a link between FIC-1-mediated AMPylation and susceptibility to the pathogen Pseudomonas aeruginosa, establishing a connection between AMPylation and innate immunity in C. elegans. |
| Volume | 12(5) |
| Pages | e1006023 |
| Published | 2016-5-1 |
| DOI | 10.1371/journal.pgen.1006023 |
| PII | PGENETICS-D-15-02363 |
| PMID | 27138431 |
| PMC | PMC4854385 |
| MeSH | Animals Caenorhabditis elegans / chemistry Caenorhabditis elegans / genetics* Caenorhabditis elegans / microbiology Caenorhabditis elegans Proteins / genetics* Carrier Proteins / chemistry Carrier Proteins / genetics Crystallography, X-Ray DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism HSP70 Heat-Shock Proteins / chemistry HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism Heat-Shock Response / genetics Histones / genetics Histones / metabolism Humans Immunity, Innate / genetics Membrane Proteins / chemistry Membrane Proteins / genetics Nucleotidyltransferases / genetics* Peptide Chain Elongation, Translational* Protein Conformation Pseudomonas aeruginosa / genetics Pseudomonas aeruginosa / pathogenicity Recombinant Proteins / chemistry Recombinant Proteins / genetics* Recombinant Proteins / metabolism |
| IF | 5.175 |
| Times Cited | 16 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2482 |