RRC ID 53293
著者 Stavoe AK, Hill SE, Hall DH, Colón-Ramos DA.
タイトル KIF1A/UNC-104 Transports ATG-9 to Regulate Neurodevelopment and Autophagy at Synapses.
ジャーナル Dev Cell
Abstract Autophagy is a cellular degradation process important for neuronal development and survival. Neurons are highly polarized cells in which autophagosome biogenesis is spatially compartmentalized. The mechanisms and physiological importance of this spatial compartmentalization of autophagy in the neuronal development of living animals are not well understood. Here we determine that, in Caenorhabditis elegans neurons, autophagosomes form near synapses and are required for neurodevelopment. We first determine, through unbiased genetic screens and systematic genetic analyses, that autophagy is required cell autonomously for presynaptic assembly and for axon outgrowth dynamics in specific neurons. We observe autophagosome biogenesis in the axon near synapses, and this localization depends on the synaptic vesicle kinesin, KIF1A/UNC-104. KIF1A/UNC-104 coordinates localized autophagosome formation by regulating the transport of the integral membrane autophagy protein, ATG-9. Our findings indicate that autophagy is spatially regulated in neurons through the transport of ATG-9 by KIF1A/UNC-104 to regulate neurodevelopment.
巻・号 38(2)
ページ 171-85
公開日 2016-7-25
DOI 10.1016/j.devcel.2016.06.012
PII S1534-5807(16)30381-1
PMID 27396362
PMC PMC4961624
MeSH Animals Animals, Genetically Modified Autophagy* Autophagy-Related Proteins / genetics Autophagy-Related Proteins / metabolism* Axons / physiology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Polarity Membrane Proteins / genetics Membrane Proteins / metabolism* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neurogenesis / physiology* Neurons / cytology* Neurons / metabolism Synapses / physiology*
IF 10.092
引用数 58
リソース情報
線虫 tm5755 tm2508 tm1642 tm3425 tm5475 tm4475 tm2976