RRC ID 53348
Author Oh S, Kawasaki I, Park JH, Shim YH.
Title cdc-25.4, a Caenorhabditis elegans Ortholog of cdc25, Is Required for Male Mating Behavior.
Journal G3 (Bethesda)
Abstract Cell division cycle 25 (cdc25) is an evolutionarily conserved phosphatase that promotes cell cycle progression. Among the four cdc25 orthologs in Caenorhabditis elegans, we found that cdc-25.4 mutant males failed to produce outcrossed progeny. This was not caused by defects in sperm development, but by defects in male mating behavior. The cdc-25.4 mutant males showed various defects during male mating, including contact response, backing, turning, and vulva location. Aberrant turning behavior was the most prominent defect in the cdc-25.4 mutant males. We also found that cdc-25.4 is expressed in many neuronal cells throughout development. The turning defect in cdc-25.4 mutant males was recovered by cdc-25.4 transgenic expression in neuronal cells, suggesting that cdc-25.4 functions in neurons for male mating. However, the neuronal morphology of cdc-25.4 mutant males appeared to be normal, as examined with several neuronal markers. Also, RNAi depletion of wee-1.3, a C. elegans ortholog of Wee1/Myt1 kinase, failed to suppress the mating defects of cdc-25.4 mutant males. These findings suggest that, for successful male mating, cdc-25.4 does not target cell cycles that are required for neuronal differentiation and development. Rather, cdc-25.4 likely regulates noncanonical substrates in neuronal cells.
Volume 6(12)
Pages 4127-4138
Published 2016-12-7
DOI 10.1534/g3.116.036129
PII g3.116.036129
PMID 27770028
PMC PMC5144981
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Cell Cycle Proteins / genetics* Fertility / genetics Gene Expression Genetic Association Studies* Germ Cells / metabolism Male Mutation Neurons / metabolism Sexual Behavior, Animal* Spermatozoa / metabolism Transgenes
IF 2.781
C.elegans tm4088