Reference - Detail
| RRC ID | 53405 |
|---|---|
| Author | Jang H, Levy S, Flavell SW, Mende F, Latham R, Zimmer M, Bargmann CI. |
| Title | Dissection of neuronal gap junction circuits that regulate social behavior in Caenorhabditis elegans. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
A hub-and-spoke circuit of neurons connected by gap junctions controls aggregation behavior and related behavioral responses to oxygen, pheromones, and food in Caenorhabditis elegans The molecular composition of the gap junctions connecting RMG hub neurons with sensory spoke neurons is unknown. We show here that the innexin gene unc-9 is required in RMG hub neurons to drive aggregation and related behaviors, indicating that UNC-9-containing gap junctions mediate RMG signaling. To dissect the circuit in detail, we developed methods to inhibit unc-9-based gap junctions with dominant-negative unc-1 transgenes. unc-1(dn) alters a stomatin-like protein that regulates unc-9 electrical signaling; its disruptive effects can be rescued by a constitutively active UNC-9::GFP protein, demonstrating specificity. Expression of unc-1(dn) in RMG hub neurons, ADL or ASK pheromone-sensing neurons, or URX oxygen-sensing neurons disrupts specific elements of aggregation-related behaviors. In ADL, unc-1(dn) has effects opposite to those of tetanus toxin light chain, separating the roles of ADL electrical and chemical synapses. These results reveal roles of gap junctions in a complex behavior at cellular resolution and provide a tool for similar exploration of other gap junction circuits. |
| Volume | 114(7) |
| Pages | E1263-E1272 |
| Published | 2017-2-14 |
| DOI | 10.1073/pnas.1621274114 |
| PII | 1621274114 |
| PMID | 28143932 |
| PMC | PMC5320989 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Electrical Synapses / genetics Electrical Synapses / metabolism* Gap Junctions / genetics Gap Junctions / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Motor Activity / genetics Pheromones / metabolism Sensory Receptor Cells / metabolism* Signal Transduction / genetics Social Behavior* |
| IF | 9.412 |
| Times Cited | 14 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm3524 tm2738 tm3393 tm3394 tm1589 tm3829 tm1896 tm4591 tm1661 |