Reference - Detail
| RRC ID | 53414 |
|---|---|
| Author | Luo S, Horvitz HR. |
| Title | The CDK8 Complex and Proneural Proteins Together Drive Neurogenesis from a Mesodermal Lineage. |
| Journal | Curr Biol |
| Abstract |
At least some animal species can generate neurons from mesoderm or endoderm, but the underlying mechanisms remain unknown. We screened for C. elegans mutants in which the presumptive mesoderm-derived I4 neuron adopts a muscle-like cell fate. From this screen, we identified HLH-3, the C. elegans homolog of a mammalian proneural protein (Ascl1) used for in vitro neuronal reprogramming, as required for efficient I4 neurogenesis. We discovered that the CDK-8 Mediator kinase module acts together with a second proneural protein, HLH-2, and in parallel to HLH-3 to promote I4 neurogenesis. Genetic analysis revealed that CDK-8 most likely promotes I4 neurogenesis by inhibiting the CDK-7/CYH-1 (CDK7/cyclin H) kinase module of the transcription initiation factor TFIIH. Ectopic expression of HLH-2 and HLH-3 together promoted expression of neuronal features in non-neuronal cells. These findings reveal that the Mediator CDK8 kinase module can promote non-ectodermal neurogenesis and suggest that inhibiting CDK7/cyclin H might similarly promote neurogenesis. |
| Volume | 27(5) |
| Pages | 661-672 |
| Published | 2017-3-6 |
| DOI | 10.1016/j.cub.2017.01.056 |
| PII | S0960-9822(17)30091-X |
| PMID | 28238659 |
| PMC | PMC5384724 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics* Basic Helix-Loop-Helix Transcription Factors / metabolism Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cyclin-Dependent Kinase 8 / genetics* Cyclin-Dependent Kinase 8 / metabolism Gene Expression Regulation, Developmental* Mesoderm / embryology Neurogenesis / genetics* |
| IF | 9.601 |
| Times Cited | 3 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1238 tm3740 tm1768 tm1688 tm299 tm604 tm2279 tm1824 tm3105 |