論文 - 詳細
| RRC ID | 53456 |
|---|---|
| 著者 | Baldwin AT, Clemons AM, Phillips BT. |
| タイトル | Unique and redundant β-catenin regulatory roles of two Dishevelled paralogs during C. elegans asymmetric cell division. |
| ジャーナル | J Cell Sci |
| Abstract |
The Wnt/β-catenin signaling pathway is utilized across metazoans. However, the mechanism of signal transduction, especially dissociation of the β-catenin destruction complex by Dishevelled proteins, remains controversial. Here, we describe the function of the Dishevelled paralogs DSH-2 and MIG-5 in the Wnt/β-catenin asymmetry (WβA) pathway in Caenorhabditis elegans, where WβA drives asymmetric cell divisions throughout development. We find that DSH-2 and MIG-5 redundantly regulate cell fate in hypodermal seam cells. Similarly, both DSH-2 and MIG-5 are required for positive regulation of SYS-1 (a C. elegans β-catenin), but MIG-5 has a stronger effect on the polarity of SYS-1 localization. We show that MIG-5 controls cortical APR-1 (the C. elegans APC) localization. DSH-2 and MIG-5 both regulate the localization of WRM-1 (another C. elegans β-catenin), acting together as negative regulators of WRM-1 nuclear localization. Finally, we demonstrate that overexpression of DSH-2 or MIG-5 in seam cells leads to stabilization of SYS-1 in the anterior seam daughter, solidifying the Dishevelled proteins as positive regulators of SYS-1. Overall, we have further defined the role of Dishevelled in the WβA signaling pathway, and demonstrated that DSH-2 and MIG-5 regulate cell fate, β-catenin nuclear levels and the polarity of β-catenin regulation. |
| 巻・号 | 129(5) |
| ページ | 983-93 |
| 公開日 | 2016-3-1 |
| DOI | 10.1242/jcs.175802 |
| PII | jcs.175802 |
| PMID | 26795562 |
| PMC | PMC6518166 |
| MeSH | Animals Asymmetric Cell Division Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Cycle Proteins / physiology* Cell Differentiation Cytoskeletal Proteins / metabolism Dishevelled Proteins / physiology* Protein Stability Protein Transport Transcription Factors / metabolism Wnt Signaling Pathway beta Catenin / metabolism* |
| IF | 4.573 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2639 |