論文 - 詳細
| RRC ID | 53467 |
|---|---|
| 著者 | Zhang D, Dubey J, Koushika SP, Rongo C. |
| タイトル | RAB-6.1 and RAB-6.2 Promote Retrograde Transport in C. elegans. |
| ジャーナル | PLoS One |
| Abstract |
Retrograde transport is a critical mechanism for recycling certain membrane cargo. Following endocytosis from the plasma membrane, retrograde cargo is moved from early endosomes to Golgi followed by transport (recycling) back to the plasma membrane. The complete molecular and cellular mechanisms of retrograde transport remain unclear. The small GTPase RAB-6.2 mediates the retrograde recycling of the AMPA-type glutamate receptor (AMPAR) subunit GLR-1 in C. elegans neurons. Here we show that RAB-6.2 and a close paralog, RAB-6.1, together regulate retrograde transport in both neurons and non-neuronal tissue. Mutants for rab-6.1 or rab-6.2 fail to recycle GLR-1 receptors, resulting in GLR-1 turnover and behavioral defects indicative of diminished GLR-1 function. Loss of both rab-6.1 and rab-6.2 results in an additive effect on GLR-1 retrograde recycling, indicating that these two C. elegans Rab6 isoforms have overlapping functions. MIG-14 (Wntless) protein, which undergoes retrograde recycling, undergoes a similar degradation in intestinal epithelia in both rab-6.1 and rab-6.2 mutants, suggesting a broader role for these proteins in retrograde transport. Surprisingly, MIG-14 is localized to separate, spatially segregated endosomal compartments in rab-6.1 mutants compared to rab-6.2 mutants. Our results indicate that RAB-6.1 and RAB-6.2 have partially redundant functions in overall retrograde transport, but also have their own unique cellular- and subcellular functions. |
| 巻・号 | 11(2) |
| ページ | e0149314 |
| 公開日 | 2016-1-1 |
| DOI | 10.1371/journal.pone.0149314 |
| PII | PONE-D-15-55549 |
| PMID | 26891225 |
| PMC | PMC4758642 |
| MeSH | Amino Acid Sequence Animals Biological Transport Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Carrier Proteins / metabolism Endocytosis Endosomes / metabolism Gene Expression Golgi Apparatus / metabolism Intestinal Mucosa / metabolism Intracellular Signaling Peptides and Proteins Molecular Sequence Data Neurons / metabolism Protein Transport Receptors, AMPA / metabolism Sequence Alignment rab GTP-Binding Proteins / chemistry rab GTP-Binding Proteins / genetics* rab GTP-Binding Proteins / metabolism |
| IF | 2.74 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2032 tm2124 |