RRC ID 5350
著者 Endoh R, Suzuki M, Benno Y, Futai K.
タイトル Candida kashinagacola sp. nov., C. pseudovanderkliftii sp. nov. and C. vanderkliftii sp. nov., three new yeasts from ambrosia beetle-associated sources.
ジャーナル Antonie Van Leeuwenhoek
Abstract Three new yeast species, Candida kashinagacola (JCM 15019(T) = CBS 10903(T)), C. pseudovanderkliftii (JCM 15025(T) = CBS 10904(T)), and C. vanderkliftii (JCM 15029(T) = CBS 10905(T)) are described on the basis of comparison of nucleotide sequences of large subunit ribosomal DNA D1/D2 region (LSU rDNA D1/D2). The nearest assigned species of the three new species was Candida llanquihuensis. Candida kashinagacola and C. pseudovanderkliftii differed from C. llanquihuensis by 3.8% nucleotide substitution of the region, while C. vanderkliftii did by 4.4%. Three new species differed in a number of physiological and growth characteristics from any previously assigned species and from one another. A phylogenetic tree based on the sequences of LSU rDNA D1/D2 showed that these new species together with Candida sp. ST-246, Candida sp. JW01-7-11-1-4-y2, Candida sp. BG02-7-20-001A-2-1 and C. llanquihuensis form a clade near Ambrosiozyma species. The new species did not assimilate methanol as a sole source of carbon, which supported the monophyly of these non methanol-assimilating species which are closely related to the methylotrophic yeasts. Candida kashinagacola was frequently isolated from the beetle galleries of Platypus quercivorus in three different host trees (Quercus serrata, Q. laurifolia and Castanopsis cuspidata) located in the sourthern part of Kyoto, Japan, thus indicating that this species may be a primary ambrosia fungus of P. quercivorus. On the other hand, C. pseudovanderkliftii and C. vanderkliftii were isolated only from beetle galleries in Q. laurifolia. Candida vanderkliftii was isolated from beetle gallery of Platypus lewisi as well as those of P. quercivorus. Candida pseudovanderkliftii and C. vanderkliftii are assumed to be auxiliary ambrosia fungi of P. quercivorus.
巻・号 94(3)
ページ 389-402
公開日 2008-10-1
DOI 10.1007/s10482-008-9256-9
PMID 18537038
MeSH Animals Biological Evolution Candida / classification Candida / genetics Candida / isolation & purification* Candida / metabolism Coleoptera / microbiology* DNA Fingerprinting DNA, Fungal / genetics DNA, Ribosomal / genetics Ecosystem Methanol / metabolism Phenotype Phylogeny Polymerase Chain Reaction Species Specificity
IF 2.674
引用数 18
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM15019 JCM15020 JCM15021 JCM15022 JCM15023 JCM15024 JCM15025 JCM15026 JCM15027 JCM15028 JCM15029 JCM15030 JCM15031 JCM15032 JCM15033