RRC ID |
5350
|
著者 |
Endoh R, Suzuki M, Benno Y, Futai K.
|
タイトル |
Candida kashinagacola sp. nov., C. pseudovanderkliftii sp. nov. and C. vanderkliftii sp. nov., three new yeasts from ambrosia beetle-associated sources.
|
ジャーナル |
Antonie Van Leeuwenhoek
|
Abstract |
Three new yeast species, Candida kashinagacola (JCM 15019(T) = CBS 10903(T)), C. pseudovanderkliftii (JCM 15025(T) = CBS 10904(T)), and C. vanderkliftii (JCM 15029(T) = CBS 10905(T)) are described on the basis of comparison of nucleotide sequences of large subunit ribosomal DNA D1/D2 region (LSU rDNA D1/D2). The nearest assigned species of the three new species was Candida llanquihuensis. Candida kashinagacola and C. pseudovanderkliftii differed from C. llanquihuensis by 3.8% nucleotide substitution of the region, while C. vanderkliftii did by 4.4%. Three new species differed in a number of physiological and growth characteristics from any previously assigned species and from one another. A phylogenetic tree based on the sequences of LSU rDNA D1/D2 showed that these new species together with Candida sp. ST-246, Candida sp. JW01-7-11-1-4-y2, Candida sp. BG02-7-20-001A-2-1 and C. llanquihuensis form a clade near Ambrosiozyma species. The new species did not assimilate methanol as a sole source of carbon, which supported the monophyly of these non methanol-assimilating species which are closely related to the methylotrophic yeasts. Candida kashinagacola was frequently isolated from the beetle galleries of Platypus quercivorus in three different host trees (Quercus serrata, Q. laurifolia and Castanopsis cuspidata) located in the sourthern part of Kyoto, Japan, thus indicating that this species may be a primary ambrosia fungus of P. quercivorus. On the other hand, C. pseudovanderkliftii and C. vanderkliftii were isolated only from beetle galleries in Q. laurifolia. Candida vanderkliftii was isolated from beetle gallery of Platypus lewisi as well as those of P. quercivorus. Candida pseudovanderkliftii and C. vanderkliftii are assumed to be auxiliary ambrosia fungi of P. quercivorus.
|
巻・号 |
94(3)
|
ページ |
389-402
|
公開日 |
2008-10-1
|
DOI |
10.1007/s10482-008-9256-9
|
PMID |
18537038
|
MeSH |
Animals
Biological Evolution
Candida / classification
Candida / genetics
Candida / isolation & purification*
Candida / metabolism
Coleoptera / microbiology*
DNA Fingerprinting
DNA, Fungal / genetics
DNA, Ribosomal / genetics
Ecosystem
Methanol / metabolism
Phenotype
Phylogeny
Polymerase Chain Reaction
Species Specificity
|
IF |
2.674
|
引用数 |
18
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM15019
JCM15020
JCM15021
JCM15022
JCM15023
JCM15024
JCM15025
JCM15026
JCM15027
JCM15028
JCM15029
JCM15030
JCM15031
JCM15032
JCM15033 |