RRC ID 53554
著者 Feng X, Ding Y, Xian M, Xu X, Zhang R, Zhao G.
タイトル Production of optically pure d-lactate from glycerol by engineered Klebsiella pneumoniae strain.
ジャーナル Bioresour Technol
Abstract In this study, glycerol was used to produce optically pure d-lactate by engineered Klebsiella pneumoniae strain. In the recombinant strain, d-lactate dehydrogenase LdhA was overexpressed, and two genes, dhaT and yqhD for biosynthesis of main byproduct 1,3-propanediol, were knocked out. To further improve d-lactate production, the culture condition was optimized and the results demonstrated that aeration rate played an important role in d-lactate production. In microaerobic fed-batch fermentation, the engineered strain accumulated 142.1g/L optically pure d-lactate with a yield of 0.82g/g glycerol, which represented the highest d-lactate production from glycerol so far. This study showed that K. pneumoniae strain has high efficiency to convert glycerol into d-lactate and high potentiality in utilization of crude glycerol from biodiesel industry.
巻・号 172
ページ 269-275
公開日 2014-11-1
DOI 10.1016/j.biortech.2014.09.074
PII S0960-8524(14)01333-9
PMID 25270041
MeSH Fermentation Glycerol / metabolism* Klebsiella pneumoniae / metabolism* Lactic Acid / biosynthesis* Metabolic Engineering* Nitrogen Compounds Oxidation-Reduction
IF 7.539
引用数 22
リソース情報
原核生物(大腸菌)