RRC ID 53572
Author Kotte O, Volkmer B, Radzikowski JL, Heinemann M.
Title Phenotypic bistability in Escherichia coli's central carbon metabolism.
Journal Mol. Syst. Biol.
Abstract Fluctuations in intracellular molecule abundance can lead to distinct, coexisting phenotypes in isogenic populations. Although metabolism continuously adapts to unpredictable environmental changes, and although bistability was found in certain substrate-uptake pathways, central carbon metabolism is thought to operate deterministically. Here, we combine experiment and theory to demonstrate that a clonal Escherichia coli population splits into two stochastically generated phenotypic subpopulations after glucose-gluconeogenic substrate shifts. Most cells refrain from growth, entering a dormant persister state that manifests as a lag phase in the population growth curve. The subpopulation-generating mechanism resides at the metabolic core, overarches the metabolic and transcriptional networks, and only allows the growth of cells initially achieving sufficiently high gluconeogenic flux. Thus, central metabolism does not ensure the gluconeogenic growth of individual cells, but uses a population-level adaptation resulting in responsive diversification upon nutrient changes.
Volume 10
Pages 736
Published 2014-7-1
DOI 10.15252/msb.20135022
PMID 24987115
PMC PMC4299493
MeSH Adaptation, Physiological Anti-Bacterial Agents Carbon / metabolism* Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial Glucose / metabolism* Phenotype Stress, Physiological
IF 9.8
Resource
Prokaryotes E. coli