論文 - 詳細
| RRC ID | 53572 |
|---|---|
| 著者 | Kotte O, Volkmer B, Radzikowski JL, Heinemann M. |
| タイトル | Phenotypic bistability in Escherichia coli's central carbon metabolism. |
| ジャーナル | Mol Syst Biol |
| Abstract |
Fluctuations in intracellular molecule abundance can lead to distinct, coexisting phenotypes in isogenic populations. Although metabolism continuously adapts to unpredictable environmental changes, and although bistability was found in certain substrate-uptake pathways, central carbon metabolism is thought to operate deterministically. Here, we combine experiment and theory to demonstrate that a clonal Escherichia coli population splits into two stochastically generated phenotypic subpopulations after glucose-gluconeogenic substrate shifts. Most cells refrain from growth, entering a dormant persister state that manifests as a lag phase in the population growth curve. The subpopulation-generating mechanism resides at the metabolic core, overarches the metabolic and transcriptional networks, and only allows the growth of cells initially achieving sufficiently high gluconeogenic flux. Thus, central metabolism does not ensure the gluconeogenic growth of individual cells, but uses a population-level adaptation resulting in responsive diversification upon nutrient changes. |
| 巻・号 | 10(7) |
| ページ | 736 |
| 公開日 | 2014-7-1 |
| DOI | 10.15252/msb.20135022 |
| PMID | 24987115 |
| PMC | PMC4299493 |
| MeSH | Adaptation, Physiological Anti-Bacterial Agents Carbon / metabolism* Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial Glucose / metabolism* Phenotype Stress, Physiological |
| IF | 8.991 |
| 引用数 | 124 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |