RRC ID 53655
Author Barton NR, Burgard AP, Burk MJ, Crater JS, Osterhout RE, Pharkya P, Steer BA, Sun J, Trawick JD, Van Dien SJ, Yang TH, Yim H.
Title An integrated biotechnology platform for developing sustainable chemical processes.
Journal J Ind Microbiol Biotechnol
Abstract Genomatica has established an integrated computational/experimental metabolic engineering platform to design, create, and optimize novel high performance organisms and bioprocesses. Here we present our platform and its use to develop E. coli strains for production of the industrial chemical 1,4-butanediol (BDO) from sugars. A series of examples are given to demonstrate how a rational approach to strain engineering, including carefully designed diagnostic experiments, provided critical insights about pathway bottlenecks, byproducts, expression balancing, and commercial robustness, leading to a superior BDO production strain and process.
Volume 42(3)
Pages 349-60
Published 2015-3-1
DOI 10.1007/s10295-014-1541-1
PMID 25416472
MeSH Biotechnology / methods* Butylene Glycols / metabolism Carbon Isotopes Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli / metabolism Fermentation Green Chemistry Technology* Metabolic Engineering Metabolic Networks and Pathways / genetics Systems Biology
IF 3.103
Times Cited 28
Prokaryotes E. coli