RRC ID 53663
Author Zhang Y, Mandava CS, Cao W, Li X, Zhang D, Li N, Zhang Y, Zhang X, Qin Y, Mi K, Lei J, Sanyal S, Gao N.
Title HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions.
Journal Nat Struct Mol Biol
Abstract Adverse cellular conditions often lead to nonproductive translational stalling and arrest of ribosomes on mRNAs. Here, we used fast kinetics and cryo-EM to characterize Escherichia coli HflX, a GTPase with unknown function. Our data reveal that HflX is a heat shock-induced ribosome-splitting factor capable of dissociating vacant as well as mRNA-associated ribosomes with deacylated tRNA in the peptidyl site. Structural data demonstrate that the N-terminal effector domain of HflX binds to the peptidyl transferase center in a strikingly similar manner as that of the class I release factors and induces dramatic conformational changes in central intersubunit bridges, thus promoting subunit dissociation. Accordingly, loss of HflX results in an increase in stalled ribosomes upon heat shock. These results suggest a primary role of HflX in rescuing translationally arrested ribosomes under stress conditions.
Volume 22(11)
Pages 906-13
Published 2015-11-1
DOI 10.1038/nsmb.3103
PII nsmb.3103
PMID 26458047
MeSH Cryoelectron Microscopy Escherichia coli / enzymology* Escherichia coli / metabolism* Escherichia coli / physiology Escherichia coli Proteins / chemistry Escherichia coli Proteins / metabolism* GTP-Binding Proteins / chemistry GTP-Binding Proteins / metabolism* Macromolecular Substances / ultrastructure Models, Molecular Protein Biosynthesis* Protein Conformation Ribosomes / metabolism* Ribosomes / ultrastructure Stress, Physiological
IF 11.98
Times Cited 30
Prokaryotes E. coli