| RRC ID |
53728
|
| 著者 |
Nord S, Bhatt MJ, Tükenmez H, Farabaugh PJ, Wikström PM.
|
| タイトル |
Mutations of ribosomal protein S5 suppress a defect in late-30S ribosomal subunit biogenesis caused by lack of the RbfA biogenesis factor.
|
| ジャーナル |
RNA
|
| Abstract |
The in vivo assembly of ribosomal subunits requires assistance by maturation proteins that are not part of mature ribosomes. One such protein, RbfA, associates with the 30S ribosomal subunits. Loss of RbfA causes cold sensitivity and defects of the 30S subunit biogenesis and its overexpression partially suppresses the dominant cold sensitivity caused by a C23U mutation in the central pseudoknot of 16S rRNA, a structure essential for ribosome function. We have isolated suppressor mutations that restore partially the growth of an RbfA-lacking strain. Most of the strongest suppressor mutations alter one out of three distinct positions in the carboxy-terminal domain of ribosomal protein S5 (S5) in direct contact with helix 1 and helix 2 of the central pseudoknot. Their effect is to increase the translational capacity of the RbfA-lacking strain as evidenced by an increase in polysomes in the suppressed strains. Overexpression of RimP, a protein factor that along with RbfA regulates formation of the ribosome's central pseudoknot, was lethal to the RbfA-lacking strain but not to a wild-type strain and this lethality was suppressed by the alterations in S5. The S5 mutants alter translational fidelity but these changes do not explain consistently their effect on the RbfA-lacking strain. Our genetic results support a role for the region of S5 modified in the suppressors in the formation of the central pseudoknot in 16S rRNA.
|
| 巻・号 |
21(8)
|
| ページ |
1454-68
|
| 公開日 |
2015-8-1
|
| DOI |
10.1261/rna.051383.115
|
| PII |
rna.051383.115
|
| PMID |
26089326
|
| PMC |
PMC4509935
|
| MeSH |
Bacteria / classification
Bacteria / genetics
Bacteria / growth & development*
Bacteria / metabolism
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Genes, Lethal
Models, Molecular
Mutation
Protein Structure, Secondary
RNA, Bacterial / metabolism
RNA, Ribosomal, 16S / metabolism*
Ribosomal Proteins / chemistry
Ribosomal Proteins / genetics
Ribosomal Proteins / metabolism*
Ribosome Subunits, Small, Bacterial / metabolism
|
| IF |
4.32
|
| 引用数 |
2
|
| リソース情報 |
| 原核生物(大腸菌) |
|
