RRC ID 53750
Author Liu S, Skory C, Qureshi N, Hughes S.
Title The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance.
Journal J. Ind. Microbiol. Biotechnol.
Abstract The yajC gene (Lbuc_0921) from Lactobacillus buchneri NRRL B-30929 was identified from previous proteomics analyses in response to ethanol treatment. The YajC protein expression was increased by 15-fold in response to 10 % ethanol vs 0 % ethanol. The yajC gene encodes the smaller subunit of the preprotein translocase complex, which interacts with membrane protein SecD and SecF to coordinate protein transport and secretion across cytoplasmic membrane in Escherichia coli. The YajC protein was linked to sensitivity to growth temperatures in E. coli, involved in translocation of virulence factors during Listeria infection, and stimulating a T cell-mediated response of Brucella abortus. In this study, the L. buchneri yajC gene was over-expressed in E. coli. The strain carrying pET28byajC that produces YajC after isopropyl β-D-1-thiogalactopyranoside induction showed tolerance to 4 % ethanol in growth media, compared to the control carrying pET28b. This is the first report linking YajC to ethanol stress and tolerance.
Volume 43(4)
Pages 441-50
Published 2016-4
DOI 10.1007/s10295-015-1730-6
PII 10.1007/s10295-015-1730-6
PMID 26790414
MeSH Bacterial Proteins / biosynthesis Bacterial Proteins / genetics* Bacterial Proteins / metabolism* Cell Membrane / drug effects Cell Membrane / metabolism Escherichia coli / cytology Escherichia coli / drug effects* Escherichia coli / genetics* Escherichia coli / metabolism Escherichia coli Proteins / biosynthesis Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Ethanol / pharmacology* Genes, Bacterial Lactobacillus / genetics* Protein Transport / drug effects SEC Translocation Channels / biosynthesis SEC Translocation Channels / genetics SEC Translocation Channels / metabolism Stress, Physiological / drug effects* Stress, Physiological / genetics
IF 3.103
Resource
Prokaryotes E. coli