| RRC ID |
53750
|
| 著者 |
Liu S, Skory C, Qureshi N, Hughes S.
|
| タイトル |
The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance.
|
| ジャーナル |
J Ind Microbiol Biotechnol
|
| Abstract |
The yajC gene (Lbuc_0921) from Lactobacillus buchneri NRRL B-30929 was identified from previous proteomics analyses in response to ethanol treatment. The YajC protein expression was increased by 15-fold in response to 10 % ethanol vs 0 % ethanol. The yajC gene encodes the smaller subunit of the preprotein translocase complex, which interacts with membrane protein SecD and SecF to coordinate protein transport and secretion across cytoplasmic membrane in Escherichia coli. The YajC protein was linked to sensitivity to growth temperatures in E. coli, involved in translocation of virulence factors during Listeria infection, and stimulating a T cell-mediated response of Brucella abortus. In this study, the L. buchneri yajC gene was over-expressed in E. coli. The strain carrying pET28byajC that produces YajC after isopropyl β-D-1-thiogalactopyranoside induction showed tolerance to 4 % ethanol in growth media, compared to the control carrying pET28b. This is the first report linking YajC to ethanol stress and tolerance.
|
| 巻・号 |
43(4)
|
| ページ |
441-50
|
| 公開日 |
2016-4-1
|
| DOI |
10.1007/s10295-015-1730-6
|
| PII |
10.1007/s10295-015-1730-6
|
| PMID |
26790414
|
| MeSH |
Bacterial Proteins / biosynthesis
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism*
Cell Membrane / drug effects
Cell Membrane / metabolism
Escherichia coli / cytology
Escherichia coli / drug effects*
Escherichia coli / genetics*
Escherichia coli / metabolism
Escherichia coli Proteins / biosynthesis
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Ethanol / pharmacology*
Genes, Bacterial
Lactobacillus / genetics*
Protein Transport / drug effects
SEC Translocation Channels / biosynthesis
SEC Translocation Channels / genetics
SEC Translocation Channels / metabolism
Stress, Physiological / drug effects*
Stress, Physiological / genetics
|
| IF |
3.103
|
| 引用数 |
10
|
| リソース情報 |
| 原核生物(大腸菌) |
JW0397-KC |
