Reference - Detail
| RRC ID | 53792 |
|---|---|
| Author | Bay DC, Stremick CA, Slipski CJ, Turner RJ. |
| Title | Secondary multidrug efflux pump mutants alter Escherichia coli biofilm growth in the presence of cationic antimicrobial compounds. |
| Journal | Res Microbiol |
| Abstract |
Escherichia coli possesses many secondary active multidrug resistance transporters (MDTs) that confer overlapping substrate resistance to a broad range of antimicrobials via proton and/or sodium motive force. It is uncertain whether redundant MDTs uniquely alter cell survival when cultures grow planktonically or as biofilms. In this study, the planktonic and biofilm growth and antimicrobial resistance of 13 E. coli K-12 single MDT gene deletion strains in minimal and rich media were determined. Antimicrobial tolerance to tetracycline, tobramycin and benzalkonium were also compared for each ΔMDT strain. Four E. coli MDT families were represented in this study: resistance nodulation and cell division members acrA, acrB, acrD, acrE, acrF and tolC; multidrug and toxin extruder mdtK; major facilitator superfamily emrA and emrB; and small multidrug resistance members emrE, sugE, mdtI and mdtJ. Deletions of multipartite efflux system genes acrB, acrE and tolC resulted in significant reductions in both planktonic and biofilm growth phenotypes and enhanced antimicrobial susceptibilities. The loss of remaining MDT genes produced similar or enhanced (acrD, acrE, emrA, emrB, mdtK, emrE and mdtJ) biofilm growth and antimicrobial resistance. ΔMDT strains with enhanced antimicrobial tolerance also enhanced biofilm biomass. These findings suggest that many redundant MDTs regulate biofilm formation and drug tolerance. |
| Volume | 168(3) |
| Pages | 208-221 |
| Published | 2017-4-1 |
| DOI | 10.1016/j.resmic.2016.11.003 |
| PII | S0923-2508(16)30146-2 |
| PMID | 27884783 |
| MeSH | Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / pharmacology* Bacterial Outer Membrane Proteins / genetics Benzalkonium Compounds / pharmacology Biofilms / drug effects* Biofilms / growth & development Cations Drug Resistance, Multiple, Bacterial* / genetics Escherichia coli / genetics* Escherichia coli / growth & development Gene Deletion* Membrane Transport Proteins / genetics* Membrane Transport Proteins / metabolism Microbial Sensitivity Tests Mutation Phenotype Plankton |
| IF | 3.217 |
| Times Cited | 12 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | Keio collection |