RRC ID 53816
Author Kuroda M, Funasaki S, Saitoh T, Sasazawa Y, Nishiyama S, Umezawa K, Simizu S.
Title Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline.
Journal FEBS Lett.
Abstract Conophylline (CNP) has various biological activities, such as insulin production. A recent study identified ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) as a direct target protein of CNP. In this study, we revealed that ARL6ip1 is a three-spanning transmembrane protein and determined the CNP-binding domain of ARL6ip1 by deletion mutation analysis of ARL6ip1 with biotinyl-amino-CNP. These results suggest that CNP is expected to be useful for future investigation of ARL6ip1 function in cells. Because of the anti-apoptotic function of ARL6ip1, CNP may be an effective therapeutic drug and/or a novel chemosensitizer for human cancers and other diseases.
Volume 587(22)
Pages 3656-60
Published 2013-11-15
DOI 10.1016/j.febslet.2013.09.017
PII S0014-5793(13)00711-4
PMID 24076029
MeSH Adaptor Proteins, Signal Transducing / chemistry* Amino Acid Sequence Binding Sites HEK293 Cells Humans Membrane Proteins / chemistry* Models, Molecular Molecular Sequence Data Plant Extracts / chemistry* Protein Binding Protein Structure, Tertiary Vinca Alkaloids / chemistry*
IF 2.999
Resource
DNA material pCI-neo/ARL6ip1(1-29)-Gluc-ARL6ip1(30-203) [V29] (RDB14554) pCI-neo/ARL6ip1(1-37)-Gluc-ARL6ip1(38-203) [L37] (RDB14555) pCI-neo/ARL6ip1(1-64)-Gluc-ARL6ip1(65-203) [P64] (RDB14556) pCI-neo/ARL6ip1(1-64)-Gluc [P64*] (RDB14557) pCI-neo/ARL6ip1(1-90)-Gluc-ARL6ip1(91-203) [R90] (RDB14558) pCI-neo/ARL6ip1(1-99)-Gluc-ARL6ip1(100-203) [T99] (RDB14559) pCI-neo/ARL6ip1(1-119)-Gluc-ARL6ip1(120-203) [A119] (RDB14560) pCI-neo/ARL6ip1(1-189)-Gluc-ARL6ip1(190-203) [R189] (RDB14561) pCI-neo/ARL6ip1(1-199)-Gluc-ARL6ip1(200-203) [E199] (RDB14562) pCI-neo/GFP-ARL6ip1(WT) (RDB14563) pCI-neo/GFP-ARL6ip1(Δ1-41) (RDB14564) pCI-neo/GFP-ARL6ip1(Δ1-86) (RDB14565) pCI-neo/GFP-ARL6ip1(Δ1-133) (RDB14566) pCI-neo/GFP-ARL6ip1(Δ42-86) (RDB14567) pCI-neo/GFP-ARL6ip1(42-133+KKNE) (RDB14568)