論文 - 詳細
| RRC ID | 53824 |
|---|---|
| 著者 | Ogawa M, Matsuda R, Takada N, Tomokiyo M, Yamamoto S, Shizukuishi S, Yamaji T, Yoshikawa Y, Yoshida M, Tanida I, Koike M, Murai M, Morita H, Takeyama H, Ryo A, Guan JL, Yamamoto M, Inoue JI, Yanagawa T, Fukuda M, Kawabe H, Ohnishi M. |
| タイトル | Molecular mechanisms of Streptococcus pneumoniae-targeted autophagy via pneumolysin, Golgi-resident Rab41, and Nedd4-1-mediated K63-linked ubiquitination. |
| ジャーナル | Cell Microbiol |
| Abstract |
Streptococcus pneumoniae is the most common causative agent of community-acquired pneumonia and can penetrate epithelial barriers to enter the bloodstream and brain. We investigated intracellular fates of S. pneumoniae and found that the pathogen is entrapped by selective autophagy in pneumolysin- and ubiquitin-p62-LC3 cargo-dependent manners. Importantly, following induction of autophagy, Rab41 was relocated from the Golgi apparatus to S. pneumoniae-containing autophagic vesicles (PcAV), which were only formed in the presence of Rab41-positive intact Golgi apparatuses. Moreover, subsequent localization and regulation of K48- and K63-linked polyubiquitin chains in and on PcAV were clearly distinguishable from each other. Finally, we found that E3 ligase Nedd4-1 was recruited to PcAV and played a pivotal role in K63-linked polyubiquitin chain (K63Ub) generation on PcAV, promotion of PcAV formation, and elimination of intracellular S. pneumoniae. These findings suggest that Nedd4-1-mediated K63Ub deposition on PcAV acts as a scaffold for PcAV biogenesis and efficient elimination of host cell-invaded pneumococci. |
| 巻・号 | 20(8) |
| ページ | e12846 |
| 公開日 | 2018-8-1 |
| DOI | 10.1111/cmi.12846 |
| PMID | 29582580 |
| MeSH | Animals Autophagy* Bacterial Proteins / metabolism Cell Line Epithelial Cells / immunology* Epithelial Cells / microbiology Humans Nedd4 Ubiquitin Protein Ligases / metabolism* Polyubiquitin / metabolism* Streptococcus pneumoniae / immunology* Streptolysins / metabolism* Ubiquitination rab GTP-Binding Proteins / metabolism* |
| IF | 3.43 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pEGFP-C1-Cog6 (RDB15979). |