RRC ID 53860
Author Virčíková V, Pokorná L, Tahotná D, Džugasová V, Balážová M, Griač P.
Title Schizosaccharomyces pombe cardiolipin synthase is part of a mitochondrial fusion protein regulated by intron retention.
Journal Biochim Biophys Acta Mol Cell Biol Lipids
Abstract Cardiolipin (CL) is a unique lipid component of mitochondria in all eukaryotes. It is important for the architecture of mitochondrial membranes and for mitochondrial dynamics. CL also creates a highly specific microenvironment of mitochondrial protein machineries. CL biosynthetic pathway is, however, only partially characterized in the fission yeast Schizosaccharomyces pombe. Here we show that CL synthase is an essential protein in S. pombe. It is encoded by the ORF SPAC22A12.08c as a C terminal part of a tandem fusion protein together with a mitochondrial hydrolase of unknown function. Expression of S. pombe CL synthase is able to complement deletion of the CRD1 gene of Saccharomyces cerevisiae and, vice versa, S. cerevisiae CRD1 gene complements deletion of S. pombe SPAC22A12.08c. The proper expression of CL synthase and its partner in the tandem protein, the mitochondrial hydrolase, is regulated at the level of alternate intron splicing. The first part of the SPAC22A12.08c fusion protein could be translated from both major SPAC22A12.08c derived mRNAs, with and without intron IV. Functional CL synthase, however, is produced only from the minor SPAC22A12.08c derived mRNA that has intron IV retained. Thus, intron retention is a novel mechanism for the differential expression of two proteins that evolved as a fusion protein and are under the control of the same promoter.
Volume 1863(10)
Pages 1331-1344
Published 2018-10-1
DOI 10.1016/j.bbalip.2018.06.019
PII S1388-1981(18)30146-X
PMID 29958934
MeSH Alternative Splicing Hydrolases / genetics* Hydrolases / metabolism Introns Membrane Proteins / genetics* Membrane Proteins / metabolism Mitochondria / genetics Mitochondria / metabolism Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Open Reading Frames Recombinant Fusion Proteins / metabolism* Schizosaccharomyces / enzymology Schizosaccharomyces / growth & development* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism
IF 4.402
Times Cited 1
DNA material S. pombe FLAG2-His SpFFH46B08 (SPW058432)