Reference - Detail
| RRC ID | 53879 |
|---|---|
| Author | Fukunaga R, Hunter T. |
| Title | MNK1, a new MAP kinase-activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates. |
| Journal | EMBO J |
| Abstract |
We have developed a novel expression screening method for identifying protein kinase substrates. In this method, a lambda phage cDNA expression library is screened by in situ, solid-phase phosphorylation using purified protein kinase and [gamma-32P]ATP. Screening a HeLa cDNA library with ERK1 MAP kinase yielded cDNAs of previously characterized ERK substrates, c-Myc and p90RSK, demonstrating the utility of this method for identifying physiological protein kinase substrates. A novel clone isolated in this screen, designated MNK1, encodes a protein-serine/threonine kinase, which is most similar to MAP kinase-activated protein kinase 2 (MAPKAP-K2), 3pK/MAPKAP-K3 and p90RSK. Bacterially expressed MNK1 was phosphorylated and activated in vitro by ERK1 and p38 MAP kinases but not by JNK/SAPK. Further, MNK1 was activated upon stimulation of HeLa cells with 12-O-tetradecanoylphorbol-13-acetate, fetal calf serum, anisomycin, UV irradiation, tumor necrosis factor-alpha, interleukin-1beta, or osmotic shock, and the activation by these stimuli was differentially inhibited by the MEK inhibitor PD098059 or the p38 MAP kinase inhibitor SB202190. Together, these results indicate that MNK1 is a novel class of protein kinase that is activated through both the ERK and p38 MAP kinase signaling pathways. |
| Volume | 16(8) |
| Pages | 1921-33 |
| Published | 1997-4-15 |
| DOI | 10.1093/emboj/16.8.1921 |
| PMID | 9155018 |
| PMC | PMC1169795 |
| MeSH | Amino Acid Sequence Animals Base Sequence Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors Calcium-Calmodulin-Dependent Protein Kinases / metabolism* Cell Line Cloning, Molecular / methods* DNA, Complementary / genetics Enzyme Activation Enzyme Inhibitors / pharmacology Flavonoids / pharmacology HeLa Cells Humans Imidazoles / pharmacology Intracellular Signaling Peptides and Proteins Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases* Molecular Sequence Data Phosphorylation Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* Pyridines / pharmacology Recombinant Fusion Proteins Sequence Homology, Amino Acid Signal Transduction / physiology Spodoptera Substrate Specificity p38 Mitogen-Activated Protein Kinases |
| IF | 9.889 |
| Times Cited | 518 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 53 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Lambda GEX5 (RDB01911) |