RRC ID 53888
Author Okabe Y, Yamaoka T, Ariizumi T, Ushijima K, Kojima M, Takebayashi Y, Sakakibara H, Kusano M, Shinozaki Y, Pulungan SI, Kubo Y, Nakano R, Ezura H.
Title Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato.
Journal Plant Cell Physiol.
Abstract Parthenocarpy, a process in which fruit set occurs without fertilization, leads to the production of seedless fruit. A number of floral homeotic mutants with abnormal stamen development exhibit parthenocarpic fruit set. Flower development is thought to repress ovary growth before anthesis. However, the mechanism of parthenocarpic fruit development caused by aberrant flower formation is poorly understood. To investigate the molecular mechanism of parthenocarpic fruit development in floral homeotic mutants, we performed functional analysis of Tomato APETALA3 (TAP3) by loss-of-function approaches. Organ-specific promoter was used to induce organ-specific loss of function in stamen and ovary/fruit. We observed increased cell expansion in tap3 mutants and TAP3-RNAi lines during parthenocarpic fruit growth. These were predominantly accompanied by the up-regulation of GA biosynthesis genes, including SlGA20ox1, SlGA20ox2, and SlGA20ox3, as well as reduced expression of the GA-inactivating gene SlGA2ox1 and the auxin signaling gene SlARF7 involved in a crosstalk between GA and auxin. These transcriptional profiles are in agreement with the GA levels in these lines. These results suggest that stamen development negatively regulates fruit set by repressing the GA biosynthesis.
Volume 60(1)
Pages 38-51
Published 2019-1-1
DOI 10.1093/pcp/pcy184
PII 5091329
PMID 30192961
MeSH Base Sequence Biosynthetic Pathways / genetics* Flowers / genetics Flowers / growth & development* Fruit / genetics Fruit / growth & development* Gene Expression Regulation, Plant Gibberellins / biosynthesis* Lycopersicon esculentum / genetics* Lycopersicon esculentum / growth & development* Mutagenesis / genetics Mutation / genetics Organ Specificity / genetics Parthenogenesis / genetics* Phenotype Plant Growth Regulators / metabolism Plant Proteins / genetics Plant Proteins / metabolism Plants, Genetically Modified Promoter Regions, Genetic / genetics RNA Interference Signal Transduction Transcription, Genetic Up-Regulation / genetics*
IF 3.929
Resource
Tomato TOMJPF00001 TOMJPF00004 TOMJPE7821-1 TOMJPE6483-1